Workshops

NMRFAM Protein Structure Determination Workshops

Date: Held annually usually during the 1^st week of June

Location: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53704.

Purpose: These introductory, hands-on workshop cover the basic steps involved in solving a protein structure by NMR and gives participants an opportunity to practice these steps in hands-on sessions led by the NMRFAM staff. Using triple-resonance NMR data collected at NMRFAM on labeled ubiquitin, the staff guides participants through the following steps of protein structure determination:

1) Description of data collection (raw data is provided)
2) Peak picking
3) Backbone Assignment
4) Sidechain Assignment
5) Automated assignments (PINE) and verification (PINE-SPARKY)
6) Three dimensional structure determination and refinement
7) Deposition of the data in BioMagResBank
8) Appendix Session : Data Processing

Participants are introduced to the newest products of NMRFAM Technology Research & Development efforts.

Format: Each section begins with a short lecture/demonstration followed by a hands-on session in which participants are given time to perform each step of protein structure determination using data provided by NMRFAM. The staff is available during the sessions to answer questions and assist participants.

Participant requirements: Workshop participants are not expected to have a prior experience with the basic steps of NMR protein structure determination. The workshop will cover the entire process and each session will build upon the previous sessions. Due to the complexity of protein structure determination, particpants will be given the data necessary to complete each session. For example, processed data will be provided for the peak picking session and fully peak-picked data will be provided for the backbone and sidechain assignment sessions. Each participant is required to bring their own laptop computer to the workshop. The laptop needs either wireless capability or a wired ethernet port. We also recommend that participants bring a mouse for their laptop.

Registration: Registration for next year’s workshop will begin in early 2010.

Please contact Dr. Anne Lynn Gillian-Daniel by email or by phone (608) 262-3173 if you have questions.