NMRFAM Bruker Pulse Programs

About this NMR pulse program library

This pulse program library provides protein and nucleic acid solution NMR experiments for use in standard methods of spectral characterization, ligand binding and structure determination.  Additional emphasis is given for isotopically enriched samples.

All pulse programs are written and tested for Bruker NMR spectrometers running Topspin or XWin-NMR 3.5, with optimized parameters for CryoProbes.

Note: This page is currently under construction, with new material being added every day.

Disclaimer

These pulse programs are provided “as is” for your information. If used incorrectly these programs may potentially damage your instrument! Users employ the pulse programs at their own risk. Neither NMRFAM nor University of Wisconsin-Madison are liable for any physical or other damage incurred during the use of the pulse programs.

How to use this Library

Each pulse program is provided as a text file.  Future versions of this library will include parameter sets and macros for particular pulse programs.

To use pulse programs, download the text file into the Bruker pulse program directory:

<path>/exp/stan/nmr/lists/pp/

where <path> is equivalent to the Topspin or XWin-NMR install directory.

The logical channels for each nucleus in these pulse programs have been encoded to circumvent discrepancies between the hardware wiring of different spectrometers.  As such, the file NUCS.incl should also be downloaded into the pulse program directory.  Once saved, edit this file for nuclei channel assignments (i.e. f1 for 1H, f2 for 13C, etc.) specific to your spectrometer.

The experiments

Pulse programs for PROTEIN samples:

Pulse programs for NUCLEIC ACID samples:

  • NA_2Dnoepr - 2D NOESY with presat
  • NA_2Dnoe11 - 2D NOESY with 1-1 jump return solvent suppression
  • NA_2Dnoew5 - 2D NOESY with Watergate 5 solvent suppression
  • NA_HNHMQC - Sensitivity enhanced 1H-15N 2D HMQC
  • NA_HCHMQC - Sensitivity enhanced 1H-13C 2D HMQC
  • NA_2Dtocsy - 2D 1H Total Correlation
  • NA_2Dcosy - 2D vicinal-1H Correlation
  • NA_2Dfenoepr - 2D NOESY for isotopic filtering/editing
  • NA_3DJmodCTHSQC - J-modulated HSQCs to measure RDCs

Adjunct .incl files for Bruker pulse programs:

  • NUCS.incl -

Useful macros for Topspin/XWin-NMR:

  • 1DXref - unified scale referencing for X-nucleus 1D spectra
  • nDXref - unified scale referencing for X-nucleus indirect dimension of nD spectra
Problems, Questions, Suggestions, Requests ?
Drop me a line: lclos@nmrfam.wisc.edu.