Date: June 9th – June 14th, 2013

Location: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544.

Purpose: This introductory, hands-on workshop will cover the basic steps involved in solving a protein structure by NMR and give participants an opportunity to practice these steps in hands-on sessions led by the NMRFAM staff. Using triple-resonance NMR data collected at NMRFAM on labeled ubiquitin, the staff will guide participants through the following steps of protein structure determination:

1) Description of data collection (raw data will be provided) 2) Peak picking 3) Backbone Assignment 4) Sidechain Assignment 5) TOCSY and NOE Assignments 6) Automated assignments 7) Three dimensional structure determination and refinement 8) Deposition of the data in BioMagResBank 9) Appendix Session : Data Processing

Due to the complexity of solving a protein structure by NMR, this workshop is intended to give participants a broad overview of the process so they can begin their own projects. It is expected that participants will require further assistance to solve a structure on their own. Participants are invited to contact NMRFAM staff in the future with any questions or problems they may have.

Format: Each section will begin with a short lecture/demonstration followed by a hands-on session in which participants will be given time to perform each step of protein structure determination using data provided by NMRFAM. The staff will be available during the sessions to answer questions and assist participants.

Participant requirements: Workshop participants are not expected to have a prior experience with basic steps of NMR protein structure determination. The workshop will cover the entire process and each session will build upon the previous sessions. Due to the complexity of protein structure determination, participants will be given the data necessary to complete each session. For example, processed data will be provided for the peak picking session and fully peak-picked data will be provided for the backbone and side-chain assignment sessions. Each participant is required to bring their own laptop computer to the workshop. The laptop needs either wireless capability or a wired ethernet port. We also recommend that participants bring a mouse for their laptop.

Registration: The fee for the workshop is $550.00 and includes all materials necessary to participate in the workshop, a reception on June 9th, a barbecue dinner on June 13th, with morning and afternoon refreshments. The last date for registration with University/Campus housing is May 23rd, 2013, and housing at Bset Western InnTowner hotel is available until May 8th, 2013. There will be no guaranteed housing after these dates. You will need to make your own arrangements for housing and parking if required for the duration of the workshop after May 23rd, 2013. Registration for the workshop will continue to be open until May 27th, 2013. Click here for registration form.  Send completed registration form to Dr. Rita Hannah

Lodging: There are 2 lodging options available and they are as follows:

We have a block of rooms that you can call (608) 233-8778 to reserve  at the Best Western InnTowner Hotel ,  located at 2424 University Ave Madison, WI 53726. It is ~1.2mile away from the workshop and on a bus route. The rooms per night are $77.00 /$99.00 for single /double occupancy and includes complimentary underground parking with free WIFI. Please ask for a room under the Group Name: NMRFAM. Rooms will be held until May 8th, 2013. Please note to obtain these rates you will need to show your State University ID.

We have also reserved a block of rooms for the NMRFAM Workshop on campus at the Vel Phillips (formerly named Friedrick) Residence Hall. The rate per night is $57.10 per person for single occupancy and $36.25 per person for double occupancy. The online reservation site for Phillips Residence Hall can be done through Housing. It provides important information regarding accommodations and services. Please contact the conference office at (608) 262-5576 or Sharon Seagren or Tom Krueger at (608) 262-3024 to make your reservations. Rooms will be held until May 23rd, 2013.

Please be aware that the Vel Phillips Residence Hall is a University dormitory and parking is not included at the Vel Phillips residence Hall. If you need parking on campus, please go to UW Transportation Services or e-mail Parking to arrange for a parking permit. Alternatively, you can call their office at 608-262-8683.

Please note that registration payment can be made using our online payment site or by check. Do not combine registration and lodging payment together. You must pay for your housing directly when making a reservation to Housing or to the Union South on your arrival.

Please contact Dr. Rita Hannah or phone (608) 262-3173 if you have questions.

Before you do anything with the SampleJet, make sure that “no NMR experiment is still actively being run by someone else”. Therefore, the rule is: “Start topspin before you operate the SampleJet” to avoid unintentionally ejecting another user’s sample while an experiment is running. If topspin won’t run due to a licensing issue, someone else is most likely still logged in and using the software. Seek advice on how to proceed from this point (see Kaifeng, Gabriel, Mark, or Milo, in whatever order works).

By default, the SampleJet is to be left in “Automatic Mode” (1.7mm Shuttle mode for Kurgan, 5mm Shuttle mode for Kerry and Rotvieh).

To change to “Manual Mode”:

Open the web browser (there is a web browser icon on the top panel after “system”)
Type in “samplejet” in the address bar (or enter http://149.236.99.55/)
Click “Login” tab on the left, click “Login” in the sub-menu
Login as “User”, with password “bruker”. System will show “Login as User successful”.
Click “Basic Service”, then “Basic Commands” in the sub-menu.
Go to the section “Change Operating Mode”, click “Manual”. This will automatically bring whatever is inside the magnet (the shuttle and sample) back into position within the SampleJet. The system will automatically restore the sample to the appropriate location.
Be patient, watch the status of the SampleJet at the bottom of the webpage until the Operating Mode displays “Manual”. You can now use the standard eject (“ej”) and inject (“ij”) commands to raise and lower the sample and spinner.

Once you are done with your experiments, you are RESPONSIBLE for removing your sample from the magnet before you exit topspin!

To change back to “Automatic Mode”:

Once your sample is removed, you MUST change the SampleJet back to automatic mode (1.7mm Shuttle mode for Kurgan, 5mm Shuttle mode for Kerry and Rotvieh).
Go to the same webpage, read the warning message in red at the bottom “Make sure magnet is empty before you leave manual mode!”. That is, you must eject your sample first.
Go to the section “Change Operating Mode”, click “1.7mm-Shuttle” for Kurgan, “5mm-Shuttle” for Kerry and Rotvieh. Wait for a while, the SampleJet will return to the default automatic mode.

WARNING: Never exit topspin with your sample in the magnet and the SampleJet in manual mode! Your PI will pay for the damage caused by your sample tube being crashed by the SampleJet inside the (cryo)probe (Probe cost ~$125,000- $150,000)!