2011 NMRFAM Protein Structure
Date: June 5th – June 10th, 2011
Location: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544.
Purpose: This introductory, hands-on workshop will cover the basic steps involved in solving a protein structure by NMR and give participants an opportunity to practice these steps in hands-on sessions led by the NMRFAM staff. Using triple-resonance NMR data collected at NMRFAM on labeled ubiquitin, the staff will guide participants through the following steps of protein structure determination:
1) Description of data collection (raw data will be provided)
2) Peak picking
3) Backbone Assignment
4) Sidechain Assignment
5) TOCSY and NOE Assignments
6) Automated assignments
7) Three dimensional structure determination and refinement
8) Deposition of the data in BioMagResBank
9) Appendix Session : Data Processing
Due to the complexity of solving a protein structure by NMR, this workshop is intended to give particpants a broad overview of the process so they can begin their own projects. It is expected that particpants will require further assistance to solve a structure on their own. Participants are invited to contact NMRFAM staff in the future with any questions or problems they may have.
Format: Each section will begin with a short lecture/demonstration followed by a hands-on session in which participants will be given time to perform each step of protein structure determination using data provided by NMRFAM. The satff will be available during the sessions to answer questions and assist participants.
Participant requirements: Workshop participants are not expected to have a prior experience with the basic steps of NMR protein structure determination. The workshop will cover the entire process and each session will build upon the previous sessions. Due to the complexity of protein structure determination, particpants will be given the data necessary to complete each session. For example, processed data will be provided for the peak picking session and fully peak-picked data will be provided for the backbone and sidechain assignment sessions. Each participant is required to bring their own laptop computer to the workshop. The laptop needs either wireless capability or a wired ethernet port. We also recommend that participants bring a mouse for their laptop.
Please contact Dr. Rita Hannah by email or by phone (608) 262-3173 if you have questions.