Purpose: This introductory, hands-on workshop will cover the basic steps involved in solving a protein structure by NMR and give participants an opportunity to practice these steps in hands-on sessions led by the NMRFAM staff. Using triple-resonance NMR data collected at NMRFAM on labeled ubiquitin, the staff will guide participants through the following steps of protein structure determination:
1) Description of data collection (raw data will be provided)
2) Peak picking
3) Backbone Assignment
4) Sidechain Assignment
5) TOCSY and NOE Assignments
6) Automated assignments
7) Three dimensional structure determination and refinement
8) Deposition of the data in BioMagResBank
9) Appendix Session : Data Processing
Due to the complexity of solving a protein structure by NMR, this workshop is intended to give particpants a broad overview of the process so they can begin their own projects. It is expected that particpants will require further assistance to solve a structure on their own. Participants are invited to contact NMRFAM staff in the future with any questions or problems they may have.
Format: Each section will begin with a short lecture/demonstration followed by a hands-on session in which participants will be given time to perform each step of protein structure determination using data provided by NMRFAM. The satff will be available during the sessions to answer questions and assist participants.
Participant requirements: Workshop participants are not expected to have a prior experience with the basic steps of NMR protein structure determination. The workshop will cover the entire process and each session will build upon the previous sessions. Due to the complexity of protein structure determination, particpants will be given the data necessary to complete each session. For example, processed data will be provided for the peak picking session and fully peak-picked data will be provided for the backbone and sidechain assignment sessions. Each participant is required to bring their own laptop computer to the workshop. The laptop needs either wireless capability or a wired ethernet port. We also recommend that participants bring a mouse for their laptop.
Registration: The fee for the workshop is $500 and includes all of the materials necessary to participate in the workshop, a reception on June 1, a barbecue dinner on June 5, and morning and afternoon refreshments. Click here for registration form.
Travel and Lodging: Travel and lodging are to be arranged by each participants. We have a block of room that you can reserve from at the Best Western InnTowner (608) 233-8778 located at 2424 University Avenue. The rooms are $89 per night and includes parking. Please ask for a room in the NMRFAMÂ block. Reservations must be made prior to May 1, 2008.
We have also reserved a block of rooms on campus at the Adams Residence Hall for $26.00 per person for double occupancy and $46.75 per person for single occupancy. Parking is not included at the Adams Residence Hall so if you need parking on campus , please indicate that on your registration form and add $8/day ($48 for 6 days) to your registration fee. You will be able to pick up your parking permit from Dr. Gillian-Daniel during the first workshop session on June 1. The online reservation site for Adams Residence Hall will open March 3, 2008 and close May 2, 2008.
The NMRFAM Protein Structure Determination Workshop will be June 3-8, 2007
MMCD – Madison Metabolomics Consortium Database – a freely-available database containing information about small chemical compounds of biological interest.
New instructions for Remote Users are now available.
The new PINE server incorporates PISTACHIO (assignments), LACS (checking of carbon-13 chemical shift referencing and possible outliers), and PECAN (secondary structure determination) to yield results consistent with all three.
