3D CDCA(NCO)CAHA - (HDCD/HACA)->N->CO->CA->HA transfer experiment


3D CDCA(NCO)CAHA - sequential assignment of prolines and poly-proline stretches in 13C/15N-labeled proteins. CryoProbe safe.

References

Bottomley et al., J. Biomol. NMR (1999) 13, 381-385.

Acquisition

Pulse program

cdcancocaha.fa

Include files etc.

Include file for optional expt time correction: faexptcorr.incl

Shaped pulse file for CO rectangular shaped pulse: fasqua.256.jc (JCAMP-DX).
Shaped pulse file for REBURP shaped pulse: reburp.256.jc (JCAMP-DX).

Read more about the pulse shape file formats.

Parameters

Acquisition parameters: 600 MHz

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_cdcancocaha.mac

Also used (for correction of digital filter artifacts): digphs.mac


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