2D {1H}-15N heteronuclear NOESY
15N-1H sensitivity enhanced HSQC
using the gp gradient syntax.
Farrow et al., Biochemistry 33, 5984-6003 (1994)
Include file for optional expt time correction: faexptcorr.incl
Acquisition parameters:
750 MHz
Use ux2flx or
equivalent utility to convert your Bruker data to Felix "dat" format.
The NOE and the reference spectra are stored interleaved in one ser file. Use the
Felix95 macro unpacknoe.mac to separate the two data sets
for easier processing.
Each 2D can be processed using the Felix95 processing macro:
ft2d_n15noe.mac
Also used (for correction of digital filter artifacts):
digphs.mac
References
Acquisition
Pulse program
Include files etc.
Parameters
Processing