4D TROSY-HNCACO - using two separate pulses for 180 Ca/C' and optional 2H decoupling


4D TROSY-HNCACO - using two separate pulses for 180 Ca/C', minimal H2O saturation and optional 2H decoupling. This program uses the gp gradient syntax. Optimized for CryoProbe use.

References

Yang and Kay, J. Am. Chem. Soc. 1999, 121, 2571-2575.

Acquisition

Pulse program

hncacotrb.fa

Include files etc.

Include file for optional expt time correction: faexptcorr.incl

Shaped pulse file for CO/Ca rectangular shaped pulse: fasqua.256.jc (JCAMP-DX).
Shaped pulse file for EBURP-1 shaped pulse: eburp1.512.jc (JCAMP-DX).
Shaped pulse file for REBURP shaped pulse: reburp.256.jc (JCAMP-DX).

Read more about the pulse shape file formats.

Parameters

Acquisition parameters: 750 MHz

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95/98 processing macro: ft4d_hncacotrb.mac

Also used (for correction of digital filter artifacts): digphs.mac


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