3D HNCA - with Watergate reverse INEPT (FHSQC) and optional 2H decoupling


3D HNCA - with Watergate reverse INEPT (FHSQC), minimal H2O saturation and optional 2H decoupling.

References

Yamazaki et al., J.Am. Chem. Soc. 1994, 116, 6464-6465

Mori et al., J. Magn. Reson. B 108, 94-98 (1995)

Sklenar, Piotto, Leppik, and Saudek: J. Magn. Reson. A 102, 241-245 (1993)

Acquisition

Pulse program

hncaf.fa

Gradient program

Gradient program: fahncaf.r

Include files etc.

Include file for pulsed field gradient definitions: Gradfa.incl

Shaped pulse file for compensated SEDUCE-1 decoupling: seduce1c6.jc (JCAMP-DX), seduce1c6.rf (binary RF format) or seduce1c6.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 6*(1/P90) Hz from carrier)

Shaped pulse file for compensated SEDUCE-1 decoupling: seduce1c5.jc (JCAMP-DX), seduce1c5.rf (binary RF format) or seduce1c5.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 5*(1/P90) Hz from carrier)

Read more about the pulse shape file formats.

Parameters

Acquisition parameters - 500 MHz: n/a

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_states.mac

Also used (for correction of digital filter artifacts): digphs.mac


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