3D HN(CO)CA - with Watergate reverse INEPT (FHSQC) and optional 2H decoupling


3D HN(CO)CA - with Watergate reverse INEPT (FHSQC), minimal H2O saturation and optional 2H decoupling.

References

Yamazaki et al., J.Am. Chem. Soc. 1994, 116, 6464-6465

Mori et al., J. Magn. Reson. B 108, 94-98 (1995)

Sklenar, Piotto, Leppik, and Saudek: J. Magn. Reson. A 102, 241-245 (1993)

Acquisition

Pulse program

hncocaf.fa

Include files etc.

Shaped pulse file for SEDUCE-1 decoupling: seduce1.jc (JCAMP-DX), seduce1.rf (binary RF format) or seduce1.txt (ASCII).

Read more about the pulse shape file formats.

Parameters

Acquisition parameters: 500 MHz

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_states.mac

Also used (for correction of digital filter artifacts): digphs.mac


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