3D TROSY-HNCO - with Watergate reverse INEPT (3-9-19)


3D TROSY-HNCO - with Watergate reverse INEPT (3-9-19) and minimal H2O saturation. This program uses the gp gradient syntax. Optimized for CryoProbe use.

References

Yang and Kay, J. Biomol. NMR. 13, 3-10 (1999) (experiment 2b in this paper) with modifications from Yang and Kay, J. Biomol. NMR. 14, 273-276 (1999).

Acquisition

Pulse program

hncotrk2b.fa

Include files etc.

Include file for optional expt time correction: faexptcorr.incl

Shaped pulse file for Ca rectangular shaped pulse: fasqua.256.jc (JCAMP-DX).
Shaped pulse file for EBURP-1 shaped pulse: eburp1.512.jc (JCAMP-DX).

Read more about the pulse shape file formats.

Parameters

Acquisition parameters: 750 MHz

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95/98 processing macro: ft3d_hncotrk2b.mac

Also used (for correction of digital filter artifacts): digphs.mac


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