3D 15N-NOESY-FHSQC, with optional 13C decoupling and SEDUCE-1 decoupling


3D 1H-15N-1H NOESY Fast HSQC with minimal water saturation. Use optional 13C decoupling and SEDUCE-1 decoupling with 13C labeled samples. This program uses the gp gradient syntax. Optimized for CryoProbe use.

References

Talluri and Wagner, J. Magn. Reson. B 112, 200-205 (1996)

Mori et al., J. Magn. Reson. B 108, 94-98 (1995)

Sklenar, Piotto, Leppik, and Saudek: J. Magn. Reson. A 102, 241-245 (1993)

Acquisition

Pulse program

n15noefhsqc.fa

Include files etc.

Include file for optional expt time correction: faexptcorr.incl

Composite pulse decoupling program for SEDUCE-1 decoupling: waltz16sp3

Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c6.jc (JCAMP-DX), seduce1c6.rf (binary RF format) or seduce1c6.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 6*(1/P90) Hz from carrier)

Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c5.jc (JCAMP-DX), seduce1c5.rf (binary RF format) or seduce1c5.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 5*(1/P90) Hz from carrier)

Read more about the pulse shape file formats.

Parameters

Acquisition parameters - 500 MHz: n15noefhsqc_500.par

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_noefhsqc.mac
Felix95 processing macro with Linear Prediction extrapolation: ft3d_noefhsqc_lp.mac

Also used (for correction of digital filter artifacts): digphs.mac


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