3D 15N-TOCSY-FHSQC, with optional 13C decoupling and SEDUCE-1 decoupling


3D TOCSY-15N-1H Fast HSQC with minimal water saturation. Use optional 13C decoupling and SEDUCE-1 decoupling with 13C labeled samples. Isotropic mixing by DIPSI2, DIPSI2RC, or DIPSI3 sequence. This program uses the gp gradient syntax. Optimized for CryoProbe use.

References

Talluri and Wagner, J. Magn. Reson. B 112, 200-205 (1996)

Mori et al., J. Magn. Reson. B 108, 94-98 (1995)

Sklenar, Piotto, Leppik, and Saudek: J. Magn. Reson. A 102, 241-245 (1993)

Acquisition

Pulse program

n15tocfhsqc.fa

Include files etc.

Include file for isotropic mixing scheme: dipsi2f1.incl, dipsi2rcf1.incl, or dipsi3f1.incl

Include file for optional expt time correction: faexptcorr.incl

Composite pulse decoupling program for SEDUCE-1 decoupling: waltz16sp3

Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c6.jc (JCAMP-DX), seduce1c6.rf (binary RF format) or seduce1c6.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 6*(1/P90) Hz from carrier)

Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c5.jc (JCAMP-DX), seduce1c5.rf (binary RF format) or seduce1c5.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 5*(1/P90) Hz from carrier)

Read more about the pulse shape file formats.

Parameters

Acquisition parameters: 500 MHz, 600 MHz

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_tocfhsqc.mac
Felix95 processing macro with Linear Prediction extrapolation: ft3d_tocfhsqc_lp.mac

Also used (for correction of digital filter artifacts): digphs.mac


| Top of Page | Main Index |