3D 15N-TOCSY-HSQC-SE, with optional 13C decoupling


3D TOCSY-15N-1H HSQC-SE with minimal water saturation. Use optional 13C decoupling with 13C labeled samples.
Isotropic mixing by DIPSI2, DIPSI2RC, or DIPSI3 sequence.

References

Zhang et al., J. Bio. NMR, 4, 845-858 (1994)

Acquisition

Pulse program

n15tochsqcse.fa

Gradient program

Gradient program: fatochsqcse.r

Include files etc.

Include file for isotropic mixing scheme: dipsi2f1.incl, dipsi2rcf1.incl, or dipsi3f1.incl

Include file for pulsed field gradient definitions: Gradfa.incl

Parameters

Acquisition parameters - 500 MHz: n/a

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_se.mac

Also used (for correction of digital filter artifacts): digphs.mac


| Top of Page | Main Index |