3D TOCSY 13C-ct-HSQC-SE, with optional 15N and 13C' decoupling


3D TOCSY 13C-1H constant-time sensitivity enhanced HSQC with optional 13C' decoupling. Use optional 15N decoupling with 15N labeled samples.

References

Vuister and Bax, J. Magn. Reson., 98, 428-435 (1992) Schleucher et al., J. Biol. NMR, 4, 301-306 (1994)

Acquisition

Pulse program

toccthsqcse.fa

Gradient program

Gradient program: fatoccthsqcse.r

Include files etc.

Include file for pulsed field gradient definitions: Gradfa.incl

Shaped pulse file for SEDUCE-1 shaped 13C' pulse: seduce1.jc (JCAMP-DX), seduce1.rf (binary RF format) or seduce1.txt (ASCII).

Read more about the pulse shape file formats.

Parameters

Acquisition parameters - 500 MHz: n/a

Processing

Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

Felix95 processing macro: ft3d_se.mac

Also used (for correction of digital filter artifacts): digphs.mac


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