Determination of the solution three dimensional structure of chemically-synthesized native ¹¹³Cd₃S₉ domain of Lobster Metallothionein by NMR Spectroscopy

Amalia Muņoz^*, F. Holger Forsterling, C. Frank Shaw III⁺ and David H. Petering

Structure Determination: 2D-Homonuclear experiments

Amino acid sequence identification, interproton distance restraints were obtained from a series of 2D NOESY at mixing times of 100, 200, 300 ms. Backbone torsional angles (phi) from the ³J_HNa and stereoespecific assignments of the chi-1 torsion restraints from the analysis of the ³J_a,b (measured in the phase sensitive COSY and the HN-b NOEs.

Figure 2. Finger print region of the TOCSY spectrum of 3 mM Cd₃ß_C in 5 mM d₁₁-Tris-HCl at pH 7.5.