PUBLICATIONS FROM THE MARKLEY LABORATORY

1. J. L. Markley, D. H. Meadows, and O. Jardetzky, "Nuclear Magnetic Resonance Studies of Helix­Coil Transitions in Polyamino Acids," J. Mol. Biol. 27, 25­40 (1967).

2. D. H. Meadows, J. L. Markley, J. S. Cohen, and O. Jardetzky, "Nuclear Magnetic Resonance Studies of the Structure and Binding Sites of Enzymes, I. Histidine Residues," Proc. Natl. Acad. Sci. U.S.A. 58, 1307­1313 (1967).

3. J. L. Markley, I. Putter, and O. Jardetzky, "High­Resolution Nuclear Magnetic Resonance Spectra of Selectively Deuterated Staphylococcal Nuclease," Science 161, 1249­1251 (1968).

4. J. L. Markley, I. Putter, and O. Jardetzky, "Application of Nuclear Magnetic Resonance in Biochemistry: An Analysis of the Aromatic Region of the Proton Magnetic Resonance Spectrum of Staphylococcal Nuclease," Fresenius' Zh. Anal. Chem. 243, 367­374 (1968).

5. J. L. Markley, T. C. Hollocher, A.S. Brill, and O. Jardetzky, "Magnetic Resonance," Phys. Tech. Biol. Res. 2B, 117­223 (1969).

6. I. Putter, A. Barreto, J. L. Markley, and O. Jardetzky, "Nuclear Magnetic Resonance Studies of the Structure and Binding Sites of Enzymes, X. Preparation of Selectively Deuterated Analogs of Staphylococcal Nuclease," Proc. Natl. Acad. Sci. U.S.A. 64, 1396 (1969).

7. I. Putter, J. L. Markley, and O. Jardetzky, "Nuclear Magnetic Resonance Studies of the Structure and Binding Sites of Enzymes, XI. Characterization of Selectively Deuterated Analogs of Staphylococcal Nuclease," Proc. Natl. Acad. Sci. U.S.A. 65, 395­401 (1970).

8. J. L. Markley, M. N. Williams, and O. Jardetzky, "Nuclear Magnetic Resonance Studies of the Structure and Binding Sites of Enzymes, XII. A Conformational Equilibrium in Staphylococcal Nuclease Involving a Histidine Residue," Proc. Natl. Acad. Sci. U.S.A. 65, 645­651 (1970).

9. O. Jardetzky and J. L. Markley, "Nuclear Magnetic Resonance Studies of Selectively Deuterated Staphylococcal Nuclease," Farmaco, Ed. Sci. 25, 894 (1970).

10. J. L. Markley and O. Jardetzky, "Nuclear Magnetic Resonance Studies of the Structure and Binding Sites of Enzymes, XIV. Inhibitor Binding to Staphylococcal Nuclease," J. Mol. Biol. 50, 223­233 (1970).

11. J. L. Markley, W. J. Horsely, and M. P. Klein, "Spin­Lattice Relaxation Measurements in Slowly Relaxing Complex Spectra," J. Chem. Phys. 55, 3604­3605 (1971).

12. O. Jardetzky, J.L. Markley, J. Thielmann, Y. Arata, and M. N. Williams, "Tentative Sequential Model for the Unfolding and Refolding of Staphylococcal Nuclease at High pH," Cold Spring Harbor Symp. Quant. Biol. 36, 257­261 (1972).

13. J. L. Markley, "High­Resolution Proton Magnetic Resonance Spectroscopy of Selectively Deuterated Enzymes," Methods Enzymol. 26, 605­627 (1972).

14. J. L. Markley, "Nuclear Magnetic Resonance Studies of Trypsin Inhibitors. Histidines of Virgin and Modified Soybean Trypsin Inhibitors (Kunitz)," Biochemistry 12, 2245­2250 (1973).

15. J. L. Markley, "High­Resolution Proton Magnetic Resonance Studies of Two Trypsin Inhibitors: Soybean Trypsin Inhibitor (Kunitz) and Ovomucoid (Hen Egg White)," Ann. N.Y. Acad. Sci. 222, 347­373 (1973).

16. J. L. Markley and S.M. Cheung, "Differential Exchange of the C2­Hydrogens of Histidine Side Chains in Native Proteins: Proposed General Technique for the Assignment of Histidine NMR Peaks in Proteins", (Proc. Conf. Stable Isotopes in Biol., Chem. and Med., Argonne, IL, May 1973), U.S. Atomic Energy Commission CONF­ 730525, 103­118 (1973).

17. J. L. Markley, "Observation of Histidine Residues in Proteins by Means of Nuclear Magnetic Resonance Spectroscopy," Accts. Chem. Res. 8, 70­80 (1975).

18. J. L. Markley, W.R. Finkenstadt, H. Dugas, P. Leduc, and D. R. Drapeau, "Proton Magnetic Resonance Titration Curves of the Three Histidine Residues of Staphylococcal Protease," Biochemistry 14, 998­1005 (1975).

19. J. L. Markley and I. Kato, "Assignment of the Histidine Proton Magnetic Resonance Peaks of Soybean Trypsin Inhibitor (Kunitz) by a Differential Deuterium Exchange Technique," Biochemistry 14, 3234­3237 (1975).

20. J. L. Markley, "Correlation Proton Magnetic Resonance Studies at 250 MHz of Bovine Pancreatic Ribonuclease. I. Reinvestigation of Histidine Peak Assignments," Biochemistry 14, 3546­3554 (1975).

21. J. L. Markley, "Correlation Proton Magnetic Resonance Studies at 250 MHz of Bovine Pancreatic Ribonuclease. II. pH and Inhibitor Induced Conformational Changes Affecting Histidine 48 and One Tyrosine Residue," Biochemistry 14, 3554­3561 (1975).

22. J. L. Markley and W. R. Finkenstadt, "Correlation Proton Magnetic Resonance Studies at 250 MHz of Bovine Pancreatic Ribonuclease. III. Mutual Electrostatic Interaction Between Histidine Residues 12 and 119," Biochemistry 14, 3562­3566 (1975).

23. J.L. Markley, E. L. Ulrich, S. P. Berg, and D. W. Krogmann, "Nuclear Magnetic Resonance Studies of the Copper Binding Sites of Blue Copper Proteins: Oxidized, Reduced, and Apoplastocyanin," Biochemistry 14, 4428­4433 (1975).

24. J.L. Markley and M. P. Porubcan, "The Charge­Relay System of Serine Proteinases: Proton Magnetic Resonance Titration Studies of the Four Histidines of Porcine Trypsin," J. Mol. Biol. 102, 487­509 (1976).

25. J.L. Markley, E. L. Ulrich, and D.W. Krogmann, "Spinach Plastocyanin: Comparison of Reduced and Oxidized Forms by Natural Abundance Carbon­13 Nuclear Magnetic Resonance Spectroscopy," Biochem. Biophys. Res. Commun. 78, 106­114 (1977).

26. J.L. Markley and I. B. Ibañez, "Zymogen Activation in Serine Proteinases. Proton Magnetic Resonance pH Titration Studies of the Two Histidines of Bovine Chymotrypsinogen A and Chymotrypsin A_a," Biochemistry 17, 4627­4640 (1978).

27. M. A. Porubcan, D. E. Neves, S. K. Rausch, and J. L. Markley, "Active Site in Zymogens, Proton Magnetic Resonance pH Titration Curves of Histidine­57 in Porcine and Bovine Trypsinogens and in their Complexes with Bovine Pancreatic Trypsin Inhibitors (Kunitz)," Biochemistry 17, 4640­4647 (1978).

28. J. L. Markley, "Hydrogen Bonds in Serine Proteinases and their Complexes with Protein Proteinase Inhibitors. Proton NMR Studies," Biochemistry 17, 4648­4656 (1978).

29. E.L. Ulrich and J. L. Markley, "Blue Copper Proteins: Nuclear Magnetic Resonance Investigations," Coord. Chem. Rev. 27, 109­140 (1978).

30. J. L. Markley, "Catalytic Groups of Serine Proteinases. NMR Investigations," In Magnetic Resonance Studies in Biology, (R.G. Shulman, Ed.) Academic Press, New York, pp 397­461 (1979).

31. M.A. Porubcan, W.M. Westler, I.B. Ibañez, and J.L. Markley, "Diisopropylphosphoryl Serine Proteinases. Proton and Phosphorus­31 NMR pH Titration Studies," Biochemistry 18, 4108­4116 (1979).

32. M. W. Baillargeon, M. Laskowski, Jr., D. E. Neves, M. A. Porubcan, R. E. Santini, and J. L. Markley, "Soybean Trypsin Inhibitor (Kunitz) and its Complex with Trypsin. Carbon­13 Nuclear Magnetic Resonance Studies of the Reactive Site Arginine," Biochemistry 19, 5703­5710 (1980).

33. J. L. Markley, D. E. Neves, W. M. Westler, I. B. Ibañez, M. A. Porubcan, and M. W. Baillargeon, "NMR Studies of Serine Proteinases," Dev. Biochem. 10, 31­62 (1980).

34. D. C. McCain and J. L. Markley, "Phosphorus­31 Spin­Lattice Relaxation in Aqueous Orthophosphate Solutions," J. Am. Chem. Soc. 102, 5559­5565 (1980).

35. T.­M. Chan, E. L. Ulrich, and J. L. Markley, "Two Iron­Two Sulfur Ferredoxin From Spinach and Cyanobacteria. NMR Studies of Their Structures and Redox Sites," In Photosynthesis II. Electron Transport and Photophosphorylation, (G. Akoyunoglou, Ed.) Balaban Int. Science Services, Philadelphia, pp 697­704 (1981).

36. E. L. Ulrich, D. W. Krogmann, and J. L. Markley, "Cytochrome c₅₃₃: Heme Environment and Unpaired Electron Distribution as Determined by ¹H NMR Spectroscopy at 360 MHz," In Photosynthesis II. Electron Transport and Photophosphorylation, (G. Akoyunoglou, Ed.) Balaban Int. Science Services, Philadelphia, pp 583­590 (1981).

37. M. A. E. Sallam, R. L. Whistler, and J. L. Markley, "6­Chloro­3­ß­D­Erythrofuranosyl­1­Phenyl­ and ­1­p­Tolyl­Pyrazolo­[3,4­b] Quinoxaline," Carbohydr. Res. 87, 87­97 (1980).

38. M. A. E. Sallam, E. I. A. Hegazy, R. L. Whistler, and J. L. Markley, "Studies on 3,6­Anhydro­Osazones of Hexoses: Characterization and Anomeric Configuration," Carbohydr. Res. 83, C1­C5 (1980).

39. J. L. Markley, F. Travers, and C. Balny, "Lack of Evidence for a Tetrahedral Intermediate in the Hydrolysis of Nitroanilide Substrates by Serine Proteinases," Eur. J. Biochem. 120, 477­485 (1981).

40. D. C. McCain, R. Virudachalam, J. L. Markley, S.S. Abdel­Meguid, and M.G. Rossmann, "Carbon­13 Study of Southern Bean Mosaic Virus," Virology 117, 501­503 (1982).

41. W. M. Westler, J. L. Markley, and W. W. Bachovchin, "Proton NMR Spectroscopy of the Active Site Histidine of a­Lytic Proteinase. Effects of Adjacent ¹³C and ¹⁵N Labels," FEBS Lett. 138, 233­235 (1982).

42. E. L. Ulrich, D. W. Krogmann, and J. L. Markley, "Structure and Heme Environment of Ferrocytochrome c₅₅₃ from ¹H NMR Studies," J. Biol. Chem. 257, 9356­9364 (1982).

43. T. Ogino, C. Garner, J. L. Markley, and K. M. Herrmann, "Biosynthesis of Aromatic Compounds: ¹³C NMR Spectroscopy of Whole Escherichia coli Cells," Proc. Natl. Acad. Sci. U.S.A. 79, 5828­5832 (1982).

44. T.­M. Chan and J. L. Markley, "Heteronuclear (¹H, ¹³C) Two­Dimensional Chemical Shift Correlation NMR Spectroscopy of a Protein. Ferredoxin from Anabaena variabilis," J. Am. Chem. Soc. 104, 4010­4011 (1982).

45. T.­M. Chan, W. M. Westler, R. E. Santini, and J. L. Markley, "Carbon­13 NMR Subspectra of a Protein Based on the Number of Attached Protons. Ferredoxin from Anabaena variabilis," J. Am. Chem. Soc. 104, 4008­4010 (1982).

46. T. Ogino, D. H. Croll I. Kato, and J.L. Markley, "Properties of Conserved Amino Acid Residues in Tandem Homologous Protein Domains. ¹H NMR Studies of the Histidines of Chicken Ovomucoid," Biochemistry 21, 3452­3460 (1982).

47. M. Bárány, D. D. Doyle, G. Graff, W.M. Westler, and J.L. Markley, "Changes in the Natural Abundance ¹³C NMR Spectra of Intact Frog Muscle upon Storage and Caffeine­Contracture," J. Biol. Chem. 257, 2741­2743 (1982).

48. D. C. McCain, R. Virudachalam, R. E. Santini, S. S. Abdel­Meguid, and J. L. Markley, "Phosphorus­31 NMR Study of Internal Motion in the RNA of Southern Bean Mosaic Virus," Biochemistry 21, 5390­5397 (1982).

49. J. A. Blaszak, E. L. Ulrich, J. L. Markley, and D. R. McMillin, "High Resolution ¹H NMR Studies of the Nickel(II) Derivative of Azurin," Biochemistry 24, 6253­6258 (1982).

50. M. A. E. Sallam, E. I. A. Hegazy, R. L. Whistler, J. L. Markley, and D. H. Croll, "Studies on 3­Epimeric L­2­Hexulose Phenylosazones. Structure and Anomeric Configuration of the 3,6­Anhydro­Osazone Derivatives Obtained from L­xylo and L­xylo­2­Hexulose Phenylosazone," Carbohydr. Res. 102, 197­206 (1982).

51. E. L. Ulrich, W. M. Westler, and J. L. Markley, "Reassignments in the ¹H NMR Spectrum of Flavin Adenine Dinucleotide by Two­Dimensional Homonuclear Chemical Shift Correlation," Tet. Lett. 24, 473­476 (1983).

52. T.­M. Chan and J.L. Markley, "Nuclear Magnetic Resonance Studies of Two­Iron­Two­Sulfur Ferredoxins. 1. Properties of the Histidine Residues," Biochemistry 22, 5982­5987 (1983).

53. T.­M. Chan, M. A. Hermodson, E. L. Ulrich, and J. L. Markley, "Nuclear Magnetic Resonance Studies of Two­Iron­Two­Sulfur Ferredoxins. 2. Determination of the Sequence of Anabaena variabilis Ferredoxin II, Assignment of Aromatic Resonances in Proton Spectra, and Effects of Chemical Modifications," Biochemistry 22, 5988­5995 (1983).

54. T.­M. Chan and J. L. Markley, "Nuclear Magnetic Resonance Studies of Two­Iron­Two Sulfur Ferredoxins. 3. Heteronuclear (¹³C,¹H) Two­Dimensional NMR Spectra, ¹³C Peak Assignments, and ¹³C Relaxation Measurements," Biochemistry 22, 5996­6002 (1983).

55. T.­M. Chan, E. L. Ulrich, and J. L. Markley, "Nuclear Magnetic Resonance Studies of Two­Iron­Two­Sulfur Ferredoxins. 4. Interactions with Redox Partners," Biochemistry 22, 6002­6007 (1983).

56. T.­M. Chan and J.L. Markley, "Nuclear Magnetic Resonance Studies of Two­Iron­Two­Sulfur Ferredoxins. 5. Hyperfine­Shifted Peaks in ¹H and ¹³C Spectra," Biochemistry 22, 6008­6010 (1983).

57. M. Bárány, D. D. Doyle, G. Graff, W. M. Westler, and J. L. Markley, "Natural Abundance ¹³C NMR Spectra of Diseased Human Muscle," J. Mag. Reson. Med. 1, 30­43 (1984).

58. R. Virudachalam, K. Sitaraman, K. L. Heuss, J. L. Markley, and P. Argos, "Evidence for pH­Induced Release of RNA from Belladonna Mottle Virus and the Stabilizing Effect of Polyamines and Cations," Virology 130, 351­359 (1983).

59. R. Virudachalam, K. Sitaraman, K. L. Heuss, P. Argos, and J. L. Markley, "Carbon­13 and Proton Nuclear Magnetic Resonance Spectroscopy of Plant Viruses. Evidence for Protein­Nucleic Acid Interactions in Belladonna Mottle Virus and Detection of Polyamines in Turnip Yellow Mosaic Virus," Virology 130, 360­371 (1983).

60. C. L. Kojiro and J. L. Markley, "Connectivity of Proton and Carbon Spectra of Plastocyanin Established by Two­Dimensional Nuclear Magnetic Resonance," FEBS Lett. 162, 52­56 (1983).

61. J. L. Markley, W. M. Westler, T.­M. Chan, C. L. Kojiro, and E. L. Ulrich, "Two­ Dimensional NMR Approaches to the Study of Protein Structure and Function," Federation Proc. 43, 2648­2656 (1984).

62. E. L. Ulrich, E.­M. M. John, G. R. Gough, M. J. Brunden, P. T. Gilham, W. M. Westler, and J. L. Markley, "Imino Proton Assignments in the ¹H NMR Spectrum of the Lambda Phage O_R3 DNA Fragment," Biochemistry 22, 4362­4365 (1983).

63. J. L. Markley and E. L. Ulrich, "Detailed Analysis of Protein Structure and Function by NMR Spectroscopy: Survey of Resonance Assignments," Annu. Rev. Biophys. Bioeng. 13, 493­521 (1984).

64. G. I. Rhyu, W. J. Ray, Jr., and J. L. Markley, "Enzyme­Bound Intermediates in the Conversion of Glucose 1­Phosphate to Glucose 6­Phosphate by Phosphoglucomutase. Phosphorus NMR Studies," Biochemistry 23, 252­260 (1984).

65. D. C. McCain, T. C. Selig, Govindjee, and J. L. Markley, "Some Plant Leaves Have Orientation­Dependent EPR and NMR Spectra," Proc. Nat. Acad. Sci. USA 81, 748­752 (1984).

66. C. Arús, M. Bárány, W.M. Westler, and J.L. Markley, "Proton Nuclear Magnetic Resonance of Human Muscle Extracts," Clinical Physiol. Biochem. 2, 49­55 (1984).

67. C. Arús, M. Bárány, W. M. Westler, and J. L. Markley, "¹H NMR of Intact Muscle at 11 Tesla," FEBS Lett. 165, 231­237 (1984).

68. W. M. Westler, G. Ortiz­Polo, and J. L. Markley, "Two­Dimensional ¹H ­ ¹³C Chemical Shift Correlated Spectroscopy of a Protein at Natural Abundance," J. Magn. Reson. 58, 354­357 (1984).

69. C. Arús, M. Bárány, W. M. Westler, and J. L. Markley, "¹H NMR of Intact Tissues at 11.1 T," J. Magn. Reson. 57, 519­525 (1984).

70. X. Wu, W. M. Westler, and J. L. Markley, "The Assignment of Imidazolium N­¹H Peaks in the ¹H NMR Spectrum of a Protein by One and Two Dimensional NOE Experiments," J. Magn. Reson. 59, 524­529 (1984).

71. R. Virudachalam, M. Harrington, J. E. Johnson, and J. L. Markley, "¹H, ¹³C, and ³¹P Nuclear Magnetic Resonance Studies of Cowpea Mosaic Virus: Detection and Exchange of Polyamines and Dynamics of the RNA," Virology 141, 43­50 (1985).

72. G. I. Rhyu, W. J. Ray, Jr., and J. L. Markley, "Multinuclear Magnetic Resonance Studies of Metal Ion Binding Sites of Phosphoglucomutase," Biochemistry 24, 2536­ 2541 (1985).

73. R. Virudachalam, P. S. Low, P. Argos, and J. L. Markley, "Turnip Yellow Mosaic Virus and its Empty Capsid have Thermal Stabilities with Opposite pH Dependence: Differential Scanning Calorimetry and ³¹P Nuclear Magnetic Resonance Spectroscopy Studies," Virology 146, 213­220 (1985).

74. E. L. Ulrich, M. E. Girvin, W. A. Cramer, and J. L. Markley, "Location and Mobility of Ubiquinones of Different Chain Length in Artificial Membrane Vesicles," Biochemistry 24, 2501­2508 (1985).

75. G. I. Rhyu, W. J. Ray, and J. L. Markley, "The Active Site Serine Phosphate and Histidine Residues of Phosphoglucomutase: pH Titration Studies Monitored by Hydrogen­1 and Phosphorus­31 NMR Spectroscopy," Biochemistry 24, 4746­4753 (1985).

76. D. C. McCain and J. L. Markley, "Water Permeability of Chloroplast Envelope Membranes: In Vivo Measurement by Saturation­Transfer NMR," FEBS Lett. 183, 353­ 358 (1985).

77. D. H. Live, C. L. Kojiro, D. Cowburn, and J. L. Markley, "Identification of Proton NMR Signals from the Metal Ligands in Cadmium­Substituted Plastocyanin via Two­ Dimensional Multiple­Quantum Detection in the Absence of Explicitly Resolved ¹H ­ ¹¹³Cd Coupling," J. Am. Chem. Soc. 107, 3043­3045 (1985).

78. R. Virudachalam and J. L. Markley, "Nuclear Magnetic Resonance Studies of Spherical Plant Viruses," Biophys. J. 49, 33­35 (1986).

79. D.C. McCain and J. L. Markley, "A Model for Interpreting the Proton NMR Spectra of Water in Plant Leaves," Biophys. J. 48, 687­694 (1985).

80. R. Virudachalam, M. Harrington, and J. L. Markley, "Thermal Stability of Cowpea Mosaic Virus Components: Differential Scanning Calorimetry Studies," Virology 146, 138­140 (1985).

81. J.L. Markley, D. H. Croll, R. Krishnamoorthi, G. Ortiz­Polo, W. M. Westler, W. C. Bogard, Jr., and M. Laskowski, Jr., "One­ and Two­Dimensional NMR Spectral Analysis of the Consequences of Single Amino Acid Replacements in Proteins," J. Cellular Biochem. 30, 291­309 (1986).

82. R. Krishnamoorthi, J. L. Markley, M. A. Cusanovich, and C. T. Przysiecki, "Hydrogen­1 Nuclear Magnetic Resonance Investigation of Clostridium pasteurianum Rubredoxin. Location and Assignment of Resonances from the Coordinating Cysteinyl Residues," Biochemistry 25, 50­54 (1986).

83. R. Krishnamoorthi, J. L. Markley, M. A. Cusanovich, C. T. Przysiecki, and T. E. Meyer, "Hydrogen­1 Nuclear Magnetic Resonance Investigation of High Potential Iron­Sulfur Proteins from Ectothiorhodospira halophila and Ectothiorhodospira vacuolata. 1. A Comparative Study of Hyperfine Shifted Resonances," Biochemistry 25, 60­67 (1986).

84. J. L. Markley, "One­ and Two­dimensional NMR Spectroscopic Investigations of the Consequences of Amino Acid Replacements in Proteins," in Protein Engineering (Dale L. Oxender, Ed.) New York: Alan R. Liss, 15­33 (1987).

85. J. L. Markley, T.-M. Chan, R. Krishnamoorthi, and E. L. Ulrich, "Nuclear Magnetic Resonance Studies of Structure­Function Relationships in Iron­Sulfur Proteins," in Frontiers of Iron­Sulfur Protein Research, H. Matsubara et al. Eds., Japan Sci. Soc. Press, Tokyo/Springer­Verlag, Berlin, 167­181 (1986).

86. G. Ortiz­Polo, R. Krishnamoorthi, J. L. Markley, D. H. Live, D. G. Davis, and D. Cowburn, "Natural Abundance ¹⁵N NMR Spectroscopy Studies of Turkey Ovomucoid Third Domain: Assignment of Peptide ¹⁵N Resonances to the Residues at the Reactive Site Region via Proton­Detected Multiple Quantum Coherence," J. Magn. Res. 68, 303­310 (1986).

87. C. Arús, W. M. Westler, M. Bárány, and J. L. Markley, "Observation of the Terminal Methyl Group in Fatty Acids of the Linolenic Series by Means of a New ¹H NMR Pulse Sequence Providing Spectral Editing and Solvent Suppression. Application to Excised Frog Muscle and Rat Brain," Biochemistry 25, 3346­3351 (1986).

88. D. C. McCain and J. L. Markley, "Rotational Spectral Density Functions for Aqueous Sucrose: Experimental Determination Using ¹³C NMR," J. Am. Chem. Soc. 108, 4259­ 4264 (1986).

89. D. C. McCain and J. L. Markley, "The Solution Conformation of Sucrose: Concentration and Temperature Dependence," Carbohyd. Res. 152, 73­80 (1986).

90. B. Tobias and J. L. Markley, "Carbon­13 Relaxation of [¹³C­1]Acetyl­Chymotrypsin Dissolved in a Cryosolvent at Subzero Temperatures," J. Magn. Reson. 69, 381­385 (1986).

91. D. C. McCain and J. L. Markley, "Internal Motions of the Three Hydroxymethyl Groups in Aqueous Sucrose," J. Magn. Reson. 73, 244­251 (1987).

92. Zs. Zolnai, S. Macura, and J. L. Markley, "An Efficient Method for Eliminating Noise in Two­Dimensional Nuclear Magnetic Resonance Spectra," Computer Enhanced Spectroscopy 3, 141­145 (1986).

93. B. J. Stockman and J. L. Markley, "Flavodoxin from Anabaena 7120: Uniform N­15 Enrichment and Hydrogen­1, Nitrogen­15, and Phosphorus­31 NMR Investigations of the FMN Binding Site in the Reduced and Oxidized States", In Flavins and Flavoproteins, (D.E. Edmondson and D.B. McCormick, Eds.) W. de Gruyter, Berlin, pp 275­278 (1987).

94. D. C. McCain, J. Croxdale, and J. L. Markley, "Water is Allocated Differently to Chloroplasts in Sun and Shade Leaves," Plant Physiol. 86, 16­18 (1988).

95. B. J. Stockman, W. M. Westler, E. S. Mooberry, and J. L. Markley, "Flavodoxin from Anabaena 7120: Uniform N­15 Enrichment and Hydrogen­1, Nitrogen­15, and Phosphorus­31 NMR Investigations of the FMN Binding Site in the Reduced and Oxidized States," Biochemistry 27, 136­142 (1988).

96. A. T. Alexandrescu, D. A. Mills, E. L. Ulrich, M. Chinami, and J.L. Markley, "NMR Assignments of the Four Histidines of Staphylococcal Nuclease in Native and Denatured States," Biochemistry 27, 2158­2164 (1988).

97. A. D. Robertson, W. M. Westler, and J. L. Markley, "Two­Dimensional NMR Studies of Kazal Proteinase Inhibitors. 1. Sequence­Specific Assignments and Secondary Structure of Turkey Ovomucoid Third Domain," Biochemistry 27, 2519­2529 (1988)

98. G. I. Rhyu and J. L. Markley, "Two­Dimensional NMR Studies of Kazal Proteinase Inhibitors. 2. Sequence­Specific Assignments and Secondary Structure of Reactive Site­Modified Turkey Ovomucoid Third Domain," Biochemistry 27, 2529­2539 (1988).

99. D. C. McCain, E. L. Ulrich, and J. L. Markley, "Internal Motions in Staphylococcal Nuclease," J. Magn. Reson. 80, 296­305 (1988).

100. Zs. Zolnai, S. Macura, and J. L. Markley, "Compression of NMR Data: Application to Two­Dimensional NMR Spectroscopy and Imaging," J. Magn. Reson. 80, 60­70 (1988).

101. D. C. McCain, J. Croxdale and J. L. Markley, "Thermal Damage to Chloroplast Envelope Membranes," Plant Physiol. 90, 606­609 (1989).

102. E. L. Ulrich, J. L. Markley, and Y. Kyogoku, "Creation of a Nuclear Magnetic Resonance Data Repository and Literature Database," Prot. Seq. Data Anal. 2, 23­37 (1989).

103. W. M. Westler, M. Kainosho, H. Nagao, N. Tomonaga, and J.L. Markley, "Two­ Dimensional NMR Strategies for Carbon­Carbon Correlations and Sequence­Specific Assignments in Carbon­13 Labeled Proteins," J. Am. Chem. Soc. 110, 4093­4095 (1988).

104. B. J. Stockman, W. M. Westler, P. Darba, and J. L. Markley, "Detailed Analysis of Carbon­13 NMR Spin Systems in a Uniformly Carbon­13 Enriched Protein: Flavodoxin from Anabaena 7120," J. Am. Chem. Soc. 110, 4096­4097 (1988).

105. B.­H. Oh, W. M. Westler, P. Darba, and J. L. Markley, "Protein Carbon­13 Spin Systems by a Single Two­Dimensional Nuclear Magnetic Resonance Experiment," Science 240, 908­911 (1988).

106. W. M. Westler, B. J. Stockman, Y. Hosoya, Y. Miyake, M. Kainosho, and J. L. Markley, "Correlation of Carbon­13 and Nitrogen­15 Chemical Shifts in Uniformly Labeled Proteins by Heteronuclear Two­Dimensional NMR Spectroscopy," J. Am. Chem. Soc. 110, 6265­6258 (1988).

107. B. J. Stockman, M. D. Reily, W. M. Westler, E. L. Ulrich, and J. L. Markley, "Concerted Two­Dimensional NMR Approaches to Hydrogen­1, Carbon­13, and Nitrogen­15 Resonance Assignments in Proteins," Biochemistry 28, 230­236 (1989).

108. A. T. Alexandrescu, E. L. Ulrich, and J. L. Markley, "Hydrogen­1 NMR Evidence for Three Interconverting Forms of Staphylococcal Nuclease: Effects of Mutations and Solution Conditions on their Distribution," Biochemistry 28, 204­211 (1989).

109. J. L. Markley, "Two­Dimensional NMR Spectroscopy of Proteins in Solution: an Overview," Methods Enzymol. 176, 12­64 (1989).

110. A. D. Robertson and J. L. Markley, "Methods for Proton Resonance Assignment of Proteins," Biol. Magn. Reson. 9, 155­176 (1990).

111. J. Fejzo, Zs. Zolnai, S. Macura, and J. L. Markley, "Analysis of Laboratory­ Frame and Rotating­Frame Cross Relaxation in Macromolecules," J. Magn. Reson. 82, 518­528 (1989).

112. Zs. Zolnai, S. Macura, and J. L. Markley, "Spline Method for Correcting Baseplane Distortions in Two­Dimensional NMR Spectra," J. Magn. Reson. 82, 496­504 (1989).

113. C. B. Grissom and J. L. Markley, "Staphylococcal Nuclease Active Site Amino Acids: pH Dependence of Tyrosines and Arginines by ¹³C NMR and Correlation with Kinetic Studies," Biochemistry 28, 2116­2124 (1989).

114. J. Alonso, C. Arús, W. M. Westler, and J. L. Markley, "Two­Dimensional Correlated Spectroscopy (COSY) of Intact Frog Muscle. Spectral Pattern Characterization and Lactate Quantitation," Magn. Reson. Med. 11, 316­330 (1989).

115. B.­H. Oh and J. L. Markley, "Complete Carbon­13 Resonance Assignments of Tryptophan in L­Lysyl­L­Tryptophyl­L­Lysine by Single­Bond and Multiple­Bond Correlated Hydrogen­1­Carbon­13 Two­Dimensional NMR," Biopolymers 28, 1833­1837 (1989).

116. B.­H. Oh, W. M. Westler, and J. L. Markley, "Carbon­13 Spin System Directed Strategy for Assigning Cross Peaks in the COSY Fingerprint Region of a Protein," J. Am. Chem. Soc. 111, 3083­3085 (1989).

117. D. C. McCain and J. L. Markley, "More Manganese Accumulates in Maple Sun Leaves than in Shade Leaves," Plant Physiol. 90, 1417­1421 (1989).

118. E. S. Mooberry, B.­H. Oh, and J. L. Markley, "Improvement of ¹³C­¹⁵N Chemical Shift Correlation Spectroscopy by Implementing Time Proportional Phase Incrementation," J. Magn. Reson. 85, 147­149 (1989).

119. A. D. Robertson, G. I. Rhyu, W. M. Westler, and J. L. Markley, "Assignment of the Carbon­13 NMR Spectra of Virgin and Reactive­Site Modified Turkey Ovomucoid Third Domain," Biopolymers 29, 461­467 (1990).

120. B. J. Stockman and J. L. Markley, "Stable­Isotope Assisted Protein NMR Spectroscopy in Solution," Advances in Biophysical Chemistry, Vol. 1, pp. 1­46 (Bush, C. A., Ed.), JAI Press Inc., Greenwich, Connecticut (1990).

121. J. Wang, D. M. LeMaster, and J. L. Markley, "Two­Dimensional ¹H NMR Studies of Staphylococcal Nuclease: 1. Solution Structure of the (Nuclease H124L)^.Thymidine­3',5'­bisphosphate^.Ca²⁺ Ternary Complex," Biochemistry 29, 88­101 (1990).

122. J. Wang, A. P. Hinck, S. N. Loh, and J. L. Markley, "Two­Dimensional ¹H NMR Studies of Staphylococcal Nuclease: 2. Sequence­Specific Assignments of Carbon­13 and Nitrogen­15 Signals from the (Nuclease H124L)^.Thymidine­3',5'­bisphosphate^.Ca²⁺ Ternary Complex," Biochemistry 29, 102­113 (1990).

123. A. T. Alexandrescu, S. N. Loh, and J. L. Markley, "Chemical Exchange Spectroscopy Based on Carbon­13. Applications to Protein Folding and Enzymology," J. Magn. Reson. 87, 523­535 (1990).

124. S. E. Kornguth, M. E. Anderson, P. Turski, J. Sorenson, H. I. Robins, J. Cohen, A. Rappe, and J. L. Markley, "Glioblastoma Multiforme: MR Imaging at 1.5 and 9.4 T After Injection of Polylysine­DTPA­Gd in Rats," Am. J. Neuro. Radiol. 11, 313­318 (1990).

125. M. H. Zehfus, M. D. Reily, E. L. Ulrich, W. M. Westler, and J. L. Markley, "¹H, ¹³C, and ¹⁵N Resonance Assignments for a Ferrocytochrome c₅₅₃ Heme by Multinuclear NMR Spectroscopy," Arch. Biochem. Biophys. 276, 369­373 (1990).

126. J. Fejzo, Zs. Zolnai, S. Macura, and J. L. Markley, "Quantitative Evaluation of Two­Dimensional Cross­Relaxation NMR Spectra of Proteins: Interproton Distances in Turkey Ovomucoid Third Domain," J. Magn. Reson. 88, 93­110 (1990).

127. J. L. Markley, B. R. Seavey, A. T. Alexandrescu, P. Darba, A. P. Hinck, S. N. Loh, C. W. McNemar, E. S. Mooberry, B.­H. Oh, B. J. Stockman, J. Wang, W. M. Westler, M. H. Zehfus, and Zs. Zolnai, "Multinuclear Magnetic Resonance Spectroscopy of Proteins: Information Content, Data Extraction and Analysis, and Database Design," In Protein Engineering. Protein Design in Basic Research, Medicine, and Industry (M. Ikehara, T. Oshima, and K. Titani, Eds.), Springer Verlag, New York, pp 285­290 (1990).

128. B. J. Stockman and J. L. Markley, "Methods in Stable­Isotope­Assisted NMR Spectroscopy of Proteins," In Protein Structure and Engineering (O. Jardetzky, Ed.) Plenum Press, New York, pp 155­192 (1989).

129. J. Fejzo, W. M. Westler, S. Macura, and J. L. Markley, "Elimination of Cross­Relaxation Effects from Two­Dimensional Chemical Exchange Spectra of Macromolecules," J. Am. Chem. Soc 112 2574­2577 (1990).

130. A. T. Alexandrescu, A. P. Hinck, and J. L. Markley, "Coupling Between Local Structure and Global Stability of a Protein: Mutants of Staphylococcal Nuclease," Biochemistry 29, 4516­4525 (1990).

131. B.­H. Oh and J. L. Markley, "Multinuclear Magnetic Resonance Studies of the 2Fe^.2S* Ferredoxin from Anabaena sp. strain PCC 7120: 1. Hydrogen­1 Resonance Assignments and Secondary Structure in Solution of the Oxidized Form," Biochemistry 29, 3993­4004 (1990).

132. B.­H. Oh, E. S. Mooberry, and J. L. Markley, "Multinuclear Magnetic Resonance Studies of the 2Fe^.2S* Ferredoxin from Anabaena sp. strain PCC 7120: 2. Sequence­Specific Carbon­13 and Nitrogen­15 Resonance Assignments of the Oxidized Form," Biochemistry 29, 4004­4011 (1990).

133. B.­H. Oh and J. L. Markley, "Multinuclear Magnetic Resonance Studies of the 2Fe^.2S* Ferredoxin from Anabaena sp. strain PCC 7120: 3. Detection and Characterization of Hyperfine­Shifted Nitrogen­15 and Hydrogen­1 Resonances of the Oxidized Form," Biochemistry 29, 4012­4017 (1990).

134. J. Wang, A. P. Hinck, S. N. Loh, and J. L. Markley, "Two­Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from

Hydrogen­1, Carbon­13, and Nitrogen­15 Spin System Assignments of Aromatic Amino Acids in the (Nuclease H124L)^.Thymidine­3',5'­Bisphosphate^.Ca²⁺ Ternary Complex," Biochemistry 29, 4242­4253 (1990).

135. Zs. Zolnai, S. Macura, and J. L. Markley, "Hilbert Transformation of Two­ and Three­Dimensional NMR Data Reduces Computer Memory Requirements and Saves Processing Time," J. Magn. Reson. 89, 94­101 (1990).

136. L. Skjeldal, W. M. Westler, and J. L. Markley, "Detection and Characterization of Hyperfine Shifted Resonances in the Proton NMR Spectrum of Anabaena 7120 Ferredoxin at High Magnetic Fields," Arch. Biochem. Biophys. 278, 482­485 (1990).

137. Zs. Zolnai, W. M. Westler, E. L. Ulrich, and J. L. Markley, "Drafting Table and Lightbox Software for Multidimensional NMR Spectral Analysis (PIXI). The Personal Computer Workstation," J. Magn. Reson. 88, 511­522 (1990).

138.K. E. Paulsen, M. T. Stankovich, B. J. Stockman, and J. L. Markley, "Redox and Spectral Properties of Flavodoxins from Anabaena 7120," Arch. Biochem. Biophys. 280, 68­73 (1990).

139. J. Alonso, C. Arús, W. M. Westler, and J. L. Markley, "Two­Dimensional Spectra of Intact Tissue: Homonuclear Hartmann­Hahn Spectroscopy Provides Increased Sensitivity and Information Content as Compared to COSY," Magn. Reson. Med. 15, 142­151 (1990).

140. A. P. Hinck, S. N. Loh, J. Wang, and J. L. Markley, "Histidine­121 of Staphylococcal Nuclease. Correction of the H^{2 1}H NMR Assignment and Reinterpretation of the Role this Residue Plays in Conformational Heterogeneity of the Protein," J. Am. Chem. Soc. 112, 9031­9034 (1990).

141. D. C. McCain and J. L. Markley, "In vivo Study of Chloroplast Volume Regulation," Biophys. J. 61, 1207­1212 (1992).

142. D. C. McCain and J. L. Markley, "Distinguishing Chloroplast Water from Nonchloroplast Water in One­Dimensional NMR Microscope Images of Plant Leaves," J. Magn. Reson. 89, 410­414 (1990).

143. B. J. Stockman, A. M. Krezel, J. L. Markley, K. G. Leonhardt, and N. A. Straus, "Hydrogen­1, Carbon­13, and Nitrogen­15 NMR Spectroscopy of Anabaena 7120 Flavodoxin: Assignment of ß­Sheet and Flavin Binding­Site Resonances and Analysis of Protein­Flavin Interactions," Biochemistry 29 9600­9609 (1990).

144. J. Fejzo, W. M. Westler, S. Macura, and J. L. Markley, "Strategies for Eliminating Unwanted Cross­Relaxation and Coherence Transfer Effects from Two­Dimensional Chemical Exchange Spectra," J. Magn. Reson. 92, 20­29 (1991).

145. J. L. Markley, P. Darba, J. Fejzo, A. M. Krezel, S. Macura, C. W. McNemar, E. S. Mooberry, B. R. Seavey, W. M. Westler, and Zs. Zolanai, "Computational Aspects of Multinuclear NMR Spectroscopy of Proteins at NMRFAM," in NATO ASI series volume on Computational Aspects of the Study of Biological Macromolecules by Nuclear Magnetic Resonance Spectroscopy (J. C. Hoch, F. M. Poulsen, and C. Redfield, Eds.), Plenum Press, New York, pp 39­50 (1991).

146. B. J. Stockman, A. M. Krezel, J. B. Olson, E. S. Mooberry, and J. L. Markley, "Flavin Binding Site Geometry in Anabaena 7120 Flavodoxin. Progress in Determining the Flavodoxin Solution Structure," In Flavins and Flavoproteins 1990, (B. Curti, S. Ronchi, and G. Zanetti, Eds.) Walter de Gruyter, Berlin, pp 377­380 (1991).

147. A. S. Edison, W. M. Westler, and J. L. Markley, "Elucidation of Protein Spin Systems and Determination of Heteronuclear Coupling Constants by Carbon­Proton­Proton Three­Dimensional NMR," J. Magn. Reson. 92, 434­438 (1991).

148. S. N. Loh, C. W. McNemar, and J. L. Markley, "Detection and Kinetic Characterization of a Novel Proline Isomerism in Staphylococcal Nuclease by NMR Spectroscopy," In Techniques in Protein Chemistry II, (J.J. Villafranca, Ed.), Academic Press, New York, pp 275­282 (1991).

149. J. Fejzo, W. M. Westler, S. Macura, and J. L. Markley, "Elimination of Chemical Exchange Mediated Spin Diffusion from Exchange Spectra of Macromolecules. Exchange Decoupled NOESY (XD­NOESY)," J. Magn. Reson. 92, 195­202 (1991).

150. W. R. Rypniewski, D. R. Breiter, M. M. Benning G. Wesenberg, B.­H. Oh, J. L. Markley, I. Rayment, and H. M. Holden, "Crystallization and Structure Determination to 2.5 Å Resolution of the Oxidized 2Fe­2S Ferredoxin Isolated from Anabaena 7120," Biochemistry 30, 4126­4131 (1991).

151a. B. R. Seavey, E. A. Farr, W. M. Westler, and J. L. Markley, "A Relational Database for Sequence­Specific Protein NMR Data," J. Biomol. NMR 1, 217­230 (1991).

151b. J. L. Markley, E. A. Farr, and B. R. Seavey, "Appendix: Check List for Protein NMR Data to be Submitted for Publication of for Database Inclusion," J. Biomol. NMR 1, 231­236 (1991).

152. D. C. McCain, W. M. Westler, and J. L. Markley, "A Two­Dimensional Technique for Chemical Shift Resolution in One­Dimensional NMR Images," J. Magn. Reson. 93, 181­183 (1991).

153. J. Fejzo, A. M. Krezel, W. M. Westler, S. Macura, and J. L. Markley, "Direct Cross­Relaxation NOESY (D.NOESY). A Method for Removing Spin­Diffusion Cross Peaks from Two­Dimensional NOE Spectra of Macromolecules," J. Magn. Reson. 92, 651­657 (1991).

154. J. Fejzo, A. M. Krezel, W. M. Westler, S. Macura and J. L. Markley, "Refinement of the NMR Solution Structure of a Protein to Remove Distortions Arising from Neglect of Internal Motion," Biochemistry 30, 3807­3811 (1991).

155. J. L. Markley, "Stable­Isotope­Assisted Multinuclear NMR Investigations of Proteins," In Proteins - Structure, Dynamics and Design, V. Renugopalakrishnan, P. R. Carey, I. C. P. Smith, S.-G. Huang, and A. C. Storer, eds, ESCOM, Leiden, pp. 29­39 1991.

156. L. Skjeldal, W. M. Westler, B.­H. Oh, A. M. Krezel, H. M. Holden, B. L. Jacobson, I. Rayment, and J. L. Markley, "Two­Dimensional Magnetization Exchange Spectroscopy of Anabaena 7120 Ferredoxin. Nuclear Overhauser Effect and Electron Self­Exchange Cross Peaks from Amino acid Residues Surrounding the 2Fe­2S^* Cluster," Biochemistry 30, 7363­7368 (1991).

157. S. Kornguth, E. Bersu, M. E. Anderson, and J. L. Markley, "Correlation of Increased Levels of Class I MHC H­2K^k in the Placenta of Murine Trisomy 16 Conceptuses with Structural Abnormalities Revealed by Magnetic Resonance Microscopy," Tetralogy 45, 383­391 (1992).

158. L. Skjeldal, J. L. Markley, V. M. Coghlan, and L. E. Vickery, "Hydrogen­1 NMR Spectra of Vertebrate [2Fe­2S] Ferredoxins. Hyperfine Resonances Suggest Different Electron Delocalization Patterns from Plant Ferredoxins," Biochemistry 30, 9078­9083 (1991).

159. J. Wang, E. S. Mooberry, W. F. Walkenhorst, and J. L. Markley, "Solution Studies of Staphylococcal Nuclease H124L. 1. Backbone ¹H and ¹⁵N Resonances and Secondary Structure of the Unligated Enzyme as Identified by Three­Dimensional NMR Spectroscopy," Biochemistry 31, 911­920 (1992).

160. J. Wang, A. P. Hinck, S. N. Loh, D. M. LeMaster, and J. L. Markley, "Solution Studies of Staphylococcal Nuclease H124L. 2. ¹H, ¹³C, and ¹⁵N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes that Accompany Formation of the Nuclease­3',5'­Thymidine Bisphosphate­Calcium Ternary Complex," Biochemistry 31, 921­936 (1992).

161. D. C. McCain and J. L. Markley, "A Nuclear Magnetic Resonance Imaging Technique Designed for Studies of Water in Plant Leaves," J. Struct. Biol. 108, 195­201 (1992).

162. A. L. P. Houseman, B.­H. Oh, M. C. Kennedy, C. Fan, M. M. Werst, H. Beinert, J. L. Markley, and B. M. Hoffman, "^14,15N, ¹³C, ⁵⁷Fe, ^1,2H Q­band ENDOR Study of Fe­S Proteins with Clusters that Have Endogenous Sulfur Ligands," Biochemistry 31, 2073­2080 (1992).

163. J. Fejzo, W. M. Westler, J. L. Markley, S. Macura, "Complete Elimination of Spin Diffusion from Selected Resonances in Two­Dimensional Cross­Relaxation Spectra of Macromolecules by a Novel Pulse Sequence (SNOESY)," J. Am. Chem. Soc. 114, 1523­1524 (1992).

164. B. L. Jacobson, Y. K. Chae, H. Böhme, J. L. Markley, and H. M.. Holden, "Crystallization and Preliminary Analysis of Oxidized, Recombinant, Heterocyst [2Fe­2S] Ferredoxin from Anabaena 7120," Arch. Biochem. Biophys. 294, 297­281 (1992).

165. Z. Dzakula, W. M. Westler, A. S. Edison and J. L. Markley, "The "CUPID" Method of Calculating the Continuous Probability Distribution of Rotamers from NMR Data," J. Am. Chem. Soc. 114, 6195­6199 (1992).

166. Z. Dzakula, A. S. Edison, W. M. Westler, and J. L. Markley, "Analysis of c₁ Rotamer Populations from NMR Data by the CUPID Method," J. Am. Chem. Soc. 114, 6200­6207 (1992).

167. B. J. Stockman and J. L. Markley, "NMR Analysis of Ligand Binding," Current Opinion Structural Biology 2, 52­56 (1992).

168. S. Macura, J. Fejzo, C. G. Hoogstraten, W. M. Westler, and J. L. Markley, "Topological Editing of Cross­Relaxation Networks," Israel J. Chem. 32, 245­256 (1992).

169. S. T. Rao, F. Shaffie, C. Yu, K. A. Satyshur, B. J. Stockman, J. L. Markley, and M. Sundaralingam, "Structure of the Oxidized Long Chain Flavodoxin from Anabaena 7120 at 2Å Resolution," Protein Science 1, 1413­1427 (1992).

170. D. D. Gilboe, D. Kintner, M. E. Anderson, J. H. Fitzpatrick, S. E. Emoto, and J. L. Markley, "Inorganic Phosphate Compartmentation in the Normal Isolated Canine Brain," J. Neurochem. 60, 2192­2203 (1993).

171. C. Royer, A. P. Hinck, S. N. Loh, K. Prehoda, X. Peng, J. Jonas, and J. L. Markley, "Effects of Amino Acid Substitutions on the Pressure Denaturation of Staphylococcal Nuclease as Monitored by Fluorescence and NMR Spectroscopy," Biochemistry 32, 5222­5232 (1993).

172. E. S. Mooberry, A. S. Edison, F. Abildgaard, J. L. Markley, I.­J. Byeon, and M.­D. Tsai, "Modification of a Bruker AM­600 Spectrometer for Double and Triple Resonance Three­ and Four­Dimensional Experiments Illustrated with Chicken Adenylate Kinase Resonance Assignments," In Spectroscopy and Structure of Molecules and Nuclei, N. R. Johnson, W. N. Shelton, and M. El­Sayed, eds., World Scientific, Singapore, pp. 375­380, 1992.

173. M. E. Anderson, J. L. Markley, Arùs, C. and M. C. Chobanian, "Perifusion Loop­Gap Resonator NMR Probe for Aerobic Cell Suspensions," Magn. Reson. Med. 29, 563­566 (1993).

174. A. P. Hinck, W. F. Walkenhorst, W. M. Westler, S. Choe, and J. L. Markley, "Overexpression and Purification of Avian Ovomucoid Third Domains in Escherichia coli," Protein Eng. 6, 221­227 (1993).

175. S. N. Loh and J. L. Markley, "Measurement of Amide Hydrogen D/H Fractionation Factors in Proteins by NMR Spectroscopy," In Techniques in Protein Chemistry IV, (R. Angeletti, Ed.), Academic Press, New York, pp 517­524 (1993).

176. J. L. Markley and M. Kainosho, "Stable Isotope Labeling and Resonance Assignments in Larger Proteins," In NMR of Biological Macromolecules: A Practical Approach, G. C. K. Roberts, Ed., Oxford University Press, Oxford, 1993, pp 101­152.

177. R. Chylla and J. L. Markley, Simultaneous Base Point Correction and Signal Recognition in Multidimensional NMR Spectra, J. Magn. Reson., Series B 102, 148­154 (1993).

178. D. C. McCain, J. Croxdale, and J. L. Markley, "Spatial Distribution of Chloroplast Water in Acer platanoides Sun and Shade Leaves," Plant Cell and Environment 16, 727­733 (1993).

179. S. Macura, W. M. Westler, and J. L. Markley, "Two­Dimensional Exchange Spectroscopy of Proteins," Methods Enzymol. 239 106­144 (1994).

180. B. L. Jacobson, Y. K. Chae, J. L. Markley, I. Rayment, and H. M. Holden, "Molecular Structure of the Oxidized, Recombinant, Heterocyst [2Fe­2S] Ferredoxin from Anabaena 7120 Determined to 1.7 Å Resolution," Biochemistry 32, 6788­6793 (1993).

181a.J. L. Markley, A. P. Hinck, S. N. Loh, K. Prehoda, D. Truckses, W. F. Walkenhorst, and J. Wang, "Case Study of Protein Structure, Stability, and Function: NMR Investigations of the Proline Residues in Staphylococcal Nuclease," Pure & Appl. Chem. 66, 65­69 (1994).

181b.J. L. Markley, A. P. Hinck, S. N. Loh, K. Prehoda, D. Truckses, W. F. Walkenhorst, and J. Wang, "Case Study of Protein Structure, Stability, and Function: NMR Investigations of the Proline Residues in Staphylococcal Nuclease," In Protein Structure Function Relationship (Z. H. Zaidi, A. Abbasi, and D. L. Smith, Eds.), TWEL Publishers, Karachi-Doncaster-Gaithersburg, pp 193­202 (1993).

182. A. S. Edison, J. L. Markley, and F. Weinhold, "Calculations of Nuclear Spin­Spin Coupling Constants with Ab Initio Molecular Orbital Wavefunctions," J. Phys. Chem. 97, 11657­11665 (1993).

183. M. E. Cabañas, J. Alonso, C. Arús, M. E. Anderson, W. M. Westler, and J. L. Markley, "A Versatile Perifusion System for the NMR Spectroscopy of Bovine Retina. Assignment of Resonances and Effect of Ischemia," Exp Eye Res., 57 669­678 (1993).

184. A. P. Hinck, E. S. Eberhardt, and J. L. Markley, "NMR Strategy for Elucidation of Xaa­Pro Peptide Bond Configurations in Proteins: Mutants of Staphylococcal Nuclease with Altered Configuration at Proline­117," Biochemistry 32, 11810­11818 (1993).

185. R. A. Chylla, and J. L. Markley, "Improved Frequency Resolution in Constant­Time Experiments by Multidimensional Bayesian Analysis," J. Biomol. NMR 3, 515­533 (1993).

186. J. K. Hurley, Z. Salamon, T. E. Meyer, J. C. Fitch, M. A. Cusanovich, J. L. Markley, H. Cheng, B. Xia, Y. K. Chae, M. Medina, C. Gmez­Moreno, and G. Tollin, "Amino Acid Residues in Anabaena Ferredoxin Crucial to Interaction with Ferredoxin­NADP⁺ Reductase: Site­Directed Mutagenesis and Laser Flash Photolysis," Biochemistry 32, 9346­9354 (1993).

187. S. N. Loh, K. E. Prehoda, J. Wang, and J. L. Markley, "Hydrogen Exchange in Unligated and Ligated Staphylococcal Nuclease," Biochemistry 32, 11022­11028 (1993).

188. S. N. Loh, and J. L. Markley, "Hydrogen Bonding in Proteins as Studied by Amide Hydrogen D/H Fractionation Factors: Application to Staphylococcal Nuclease," Biochemistry 33, 1029­1036 (1994).

189. J. B. Olson, Jr., and J. L. Markley, "Evaluation of an Algorithm for the Automated Sequential Assignment of Protein Backbone Resonances: A Demonstration of the Connectivity Tracing Assignment Tools (CONTRAST) Software Package," J. Biomol. NMR. 4, 385­410 (1994).

190. C. G. Hoogstraten, W. M. Westler, S. Macura, and J. L Markley, "Improved Measurement of Longer Proton­Proton Distances in Proteins by Relaxation Network Editing," J. Magn. Reson., Series B, 102, 232­235 (1993).

191. A. S. Edison, J. L. Markley, and F. Weinhold, "Calculations of One­, Two, and Three­Bond Nuclear Spin­Spin Couplings in a Model Peptide and Experimental Correlations with Experimental Data," J. Biomol. NMR 4, 519­542 (1994).

192. A. S. Edison, F. Weinhold, W. M. Westler, and J. L. Markley, "Estimates of and Torsion Angles in Proteins from One­, Two­, and Three­Bond Nuclear Spin­Spin Couplings: Application to Staphylococcal Nuclease," J. Biomol. NMR 4, 543­551 (1994).

193. A. M. Krezel, P. Darba, A. D. Robertson, J. Fejzo, S. Macura, and J. L. Markley, "Solution Structure of Turkey Ovomucoid Third Domain as Determined from Nuclear Magnetic Resonance Data," J. Mol. Biol. 242, 203­214 (1994).

194. W. F. Walkenhorst, A. M. Krezel, G. I. Rhyu, and J. L. Markley, "Solution Structure of Reactive­Site Hydrolyzed Turkey Ovomucoid Third Domain by Nuclear Magnetic Resonance and Distance Geometry Methods," J. Mol. Biol. 242, 215­230 (1994).

195. A. S. Edison, F. Abildgaard, W. M. Westler, E. S. Mooberry, and J. L. Markley, "Practical Introduction to the Theory and Implementation of Multinuclear, Multidimensional NMR Experiments," Methods. Enzymol. 239, 3­79 (1994).

196. E. S. Mooberry, F. Abildgaard, and J. L. Markley, "Modifications of an Older Model NMR Console for the Collection of Multinuclear, Multidimensional Spectral Data," Methods. Enzymol. 239, 247­256 (1994).

197. S. Macura, J. Fejzo, W. M. Westler, and J. L. Markley, "Influence of Slow Internal Motion in Proteins on Cross­Relaxation Rates Determined by Two­Dimensional Exchange Spectroscopy," Bull. Magn. Reson. 16, 73­93 (1994).

198. J. K. Hurley, H. Cheng, B. Xia, J. L. Markley, M. Medina, C. Gmez­Moreno, and G. Tollin, "An Aromatic Amino Acid is Required at Position 65 in Anabaena Ferredoxin for Rapid Electron Transfer to Ferredoxin:NADP⁺ Reductase," J. Am. Chem. Soc. 115 11698­11701 (1993).

199. H. Zhao, W. M. Westler and J. L. Markley, "Precise Determination of T₁ Relaxation Values by a Method in which Pairs of Non­Equilibrium Magnetizations are Measured across a Constant Relaxation Period," J. Magn. Reson. Ser. A 112, 139­143 (1995).

200. Y. K. Chae, F. Abildgaard, E. S. Mooberry, and J. L. Markley, "Multinuclear, Multidimensional NMR Studies of Anabaena 7120 Heterocyst Ferredoxin. Sequence­Specific Resonance Assignments and Secondary Structure of the Oxidized Form in Solution," Biochemistry 33, 3287­3295 (1994).

201. H. M. Holden, B. L. Jacobson, J. K. Hurley, G. Tollin, B.­H. Oh, L. Skjeldal, Y. K. Chae, H. Cheng, B. Xia, and J. L. Markley, "Structure­Function Studies of [2Fe­2S] Ferredoxins," J. Bioeng. Biomemb. 26, 67­88 (1994).

202. I.­J. L. Byeon, H. Yan, A. S. Edison, E. S. Mooberry, F. Abildgaard, J. L. Markley, and M. D. Tsai, "Mechanism of Adenylate Kinase. ¹H, ¹³C, and ¹⁵N NMR Assignments, Secondary Structure, and Substrate Binding Sites," Biochemistry 32, 12508­12521 (1993).

203. Y. K. Chae and J. L. Markley, "Analysis of the Hyperfine­Shifted Nitrogen­15 Resonances of the Oxidized Form of Anabaena 7120 Heterocyst Ferredoxin," Biochemistry 34, 188­193 (1995).

204. H. Cheng, B. Xia, G. H. Reed, and J. L. Markley, "Optical, EPR, and ¹H NMR Spectroscopy of Serine­Ligated [2Fe­2S] Ferredoxins Produced by Site­Directed Mutagenesis of Cysteine Residues in Recombinant Anabaena 7120 Vegetative Ferredoxin," Biochemistry, 33 3155­3164 (1994).

205. J. K. Hurley, Z. Salamon, T. E. Meyer, J. C. Fitch, M. A. Cusanovich, G. Tollin, H. Cheng, B. Xia, Y. K. Chae, J. L. Markley, M. Medina, and C. Gmez­Moreno, "Structure­Function Relationships in Ferredoxin: Site­Directed Mutagenesis and Time­Resolved Absorption Spectroscopy Applied to the Ferredoxin:Ferredoxin NADP+ Reductase Interaction," In Flavins and Flavoproteins 1993 (K. Yagi, Ed.), Walter de Gruyter, Berlin, pp 435-438 (1994).

206. S. Kornguth, M. Anderson, J. L. Markley, and A. Shedlovsky, "Near­Microscopic Magnetic Resonance Imaging of the Brains of Phenylalanine Hydroxylase­Deficient Mice, Normal Littermates, and of Normal BALB/c Mice at 9.4 Tesla," Neuroimage 1, 220­229 (1994).

207. M. Ghassemian, B. Wong, F. Ferreira, J. L. Markley, and N. Straus, "Cloning, Sequencing and Transcriptional Studies of the Genes for Cytochrome c­553 and Plastocyanin from Anabaena sp. PCC 7120," Microbiology 140, 1151­1159 (1994).

208. S. N. Loh, K. E. Prehoda, J. Wang, and J. L. Markley, "Measuring Global and Local Structural Free Energy Changes in Staphylococcal Nuclease by NMR­Observed Hydrogen Exchange," In Techniques in Protein Chemistry V (J. W. Crabb, Ed.), Academic Press, New York, pp 431­438 (1994).

209. B. M. Burkhart, B. Ramakrishnan, H. Yan, R. J. Reedstrom, J. L. Markley, N. A. Straus, and M. Sundaralingam, "Molecular Structure of the Trigonal Form of Recombinant Oxidized Flavodoxin from Anabaena 7120 at 1.40 Å Resolution," Acta. Crystallogr. Ser. D. 51, 318-330 (1995).

210. Y. K. Chae, B. Xia, H. Cheng, B.­H. Oh, L. Skjeldal, W. M. Westler, and J. L. Markley, "Multinuclear Magnetic Resonance and Mutagenesis Studies of Structure­Function Relationships in [2Fe­2S] Ferredoxins," In Nuclear Magnetic Resonance of Paramagnetic Proteins (G. N., LaMar, Ed.), Nato ASI Series C, Vol. 457, Kluwer, Dordrecht, pp 297­317 (1995).

211. H. Cheng, B. Xia, Y. K. Chae, W. M. Westler, and J. L. Markley, "Magnetic Resonance Studies of Isotopically Labeled Paramagnetic Proteins: [2Fe­2S] Ferredoxins," In Stable Isotope Applications in Biomolecular Structure and Mechanisms (J. Trewhella, T. Cross, & C. J. Unkefer, Eds.), Los Alamos National Laboratory, Los Alamos, pp 171­188 (1994).

212. B. Xia, H. Cheng, L. Skjeldal, V. M. Coghlin, L. E. Vickery, and J. L. Markley, "Multinuclear Magnetic Resonance and Mutagenesis Studies of the Histidine Residues of Human Mitochondrial Ferredoxin," Biochemistry 34, 180­187 (1995).

213. H. Cheng, W. M. Westler, B. Xia, B.­H. Oh, and J. L. Markley, "Protein Expression, Selective Isotopic Labeling, and Analysis of Hyperfine­Shifted NMR Signals of Anabaena 7120 Vegetative [2Fe­2S] Ferredoxin," Arch. Biochem. Biophys 316, 619­634 (1995).

214. H. Cheng and J. L. Markley, "NMR Spectroscopic Studies of Paramagnetic Proteins: Iron­Sulfur Proteins," Ann. Rev. Biophys. Biomol. Structure 24, 209­237 (1995).

215. K. E. Prehoda and J. L. Markley, "Use of Partial Molar Volumes of Model Compounds in the Interpretation of High­Pressure Effects on Proteins," In High­Pressure Effects in Molecular Biophysics and Enzymology (J. L. Markley, C. A. Royer, and D. Northrop, Eds.), Oxford University Press, pp. 33­43 (1996).

216. R. A. Chylla and J. L. Markley, "Theory and Application of the Maximum Likelihood Principle to NMR Parameter Estimation of Multidimensional NMR Data," J. Biomol. NMR 5, 245­258 (1995).

217. J. K. Hurley, M. S. Caffrey, J. L. Markley, H. Cheng, B. Xia, Y. K. Chae, H. M. Holden, and G. Tollin, "Mutations of Surface Residues in Anabaena Vegetative and Heterocyst Ferredoxin that Affect Thermodynamic Stability as Determined by Guanidine Hydrochloride Denaturation," Protein Sci. 4, 58­64 (1995).

218. C. Gmez­Moreno, M. Medina, J. K. Hurley, M. A. Cusanovich, J. L. Markley, H. Cheng, B. Xia, Y. K. Chae, and G. Tollin, "Protein Engineering for the Elucidation of the Mechanism of Electron Transfer in Redox Proteins," Biochem. Soc. Trans. 22, 796­800 (1994).

219. J. Haginaka, T. Murashima, T. C., Pinkerton, J. P. Comiskey, E. L. Ulrich, J. L. Markley, and W. F. Walkenhorst, "Enantioselectivity of Ovomucoid Bonded­Phase HPLC Columns Produced with Isolated Domains," Kuromatogurafi 14, 100­101 (1993).

220. J. B. Pearson, J.­F. Wang, J. L. Markley, H.­b. Le, and E. Oldfield, "Protein Structure Refinement Using Carbon­13 Nuclear Magnetic Resonance Spectroscopic Chemical Shifts and Quantum Chemistry, J. Am. Chem. Soc. 117, 8823-8829 (1995).

221. Zs. Zolnai, N. Jurani, J. L. Markley, and S. Macura, "Magnetization Exchange Network Editing: Mathematical Principles and Experimental Demonstration," Chem. Phys. 200, 161-179 (1995).

222. G. J. A. Vidugiris. J. L. Markley, and C. A. Royer, "Evidence for a Molten Globule­Like Transition State in Protein Folding from Determination of Activation Volumes," Biochemistry 34, 4909­4912 (1995).

223. C. G. Hoogstraten, W. M. Westler, E. S. Mooberry, S. Macura, and J. L. Markley, "Analysis of Transverse Cross Relaxation in Proteins within a Simplified Relaxation Submatrix: BD­ROESY," J. Magn. Reson. Ser. B 109, 76-79 (1995).

224. T. C. Pinkerton, W. Jeffrey­Howe, E. L. Ulrich, J. P. Comiskey, J. Haginaka, T. Murashima, W. F. Walkenhorst, W. M. Westler, and J. L. Markley, "Protein Binding Chiral Discrimination of HPLC Stationary Phases made with Whole, Fragmented, and Third Domain Turkey Ovomucoid," Anal. Chem. 67, 2354-2367 (1995)..

225. A. S. Edison, F. Weinhold, and J. L. Markley, "Theoretical Studies of Protium/Deuterium Fractionation Factors and Cooperative Hydrogen Bonding in Peptides," J. Am. Chem. Soc. 117, 9619-9624 (1995).

226. A. S. Edison, J. L. Markley, and F. Weinhold, "Ab Initio Calculations of Protium/Deuterium Fractionation Factors in O₂H₅⁺ Clusters," J. Phys. Chem. 99, 8013­8016 (1995).

227. D. Ming, J. L. Markley, and G. Hellekant, "Quantifying Sulfhydryl Groups of Cysteinyl Residues in Peptides and Proteins," BioTechniques 18, 808­810 (1995).

228. .J. Schleucher, J. L. Markley, and T. D.Sharkey, "Hydrogen Isotope Ratios of Plant Metabolites," In Photosynthesis: from Light to Biosphere (P. Mathis, Ed.), Kluwer Academic Publishers, Vol. V, 1009-1012 (1995).

229. D. S. Wishart, C. G. Bigam, J. Yao, F. Abildgaard, H. J. Dyson, E. Oldfield, J. L. Markley, and B. D. Sykes, "¹³C and ¹⁵N Chemical Shift Referencing in Biomolecular NMR," J. Biomol. NMR. 6, 135-140 (1995).

230. B. Xia, W. M. Westler, H. Cheng, J. Meyer, J.­M. Moulis, and J. L. Markley, "Detection and Classification of Hyperfine­shifted ¹H, ²H, and ¹⁵N Resonances from the Four Cysteines that Ligate Iron in Oxidized and Reduced Clostridium pasteurianum Rubredoxin," J. Am. Chem. Soc. 117, 5347­5350 (1995).

231. C. G. Hoogstraten, W. M. Westler, S. Macura, and J. L. Markley, "NOE Measurements in the Absence of Spin Diffusion: Application to Methylene Groups in Proteins and Effects on Local Structural Parameters," J. Am. Chem. Soc. 117, 5610­5611 (1995); correction, loc. cit. p. 8885.

232. J. A. Navarro, M. Hervás, C. Genzor, G. Cheddar, M. F. Fillat, M. A. de la Rosa, C. Gómez­Moreno, H. Cheng, B. Xia, Y. K. Chae, H. Yan, B. Wong, N. Straus, J. L. Markley, J. K. Hurley, and G. Tollin, "Site­Specific Mutagenesis Demonstrates that the Structural Requirements for Efficient Electron Transfer in Anabaena Ferredoxin and Flavodoxin are Highly Dependent on the Reaction Partner: Kinetic Studies with Photosystem I, Ferredoxin:NADP+ Reductase and Cytochrome c," Arch. Biochem. Biophys. 321, 229-238 (1995).

233. C. G. Hoogstraten, S. Choe, W. M. Westler, and J. L. Markley, "Comparison of the Accuracy of Protein Solution Structures Derived from Conventional and Network­Edited NOESY Data," Protein Science 4, 2289-2299 (1995).

234. B. Xia, H. Cheng, Y. K. Chae, L. Skjeldal, W. M. Westler, and J. L. Markley, "Iron­Sulfur Proteins: Investigations of Hyperfine­Shifted Hydrogen, Carbon, and Nitrogen Resonances," In NMR as a Structural Tool For Macromolecules: Current Status and Future Directions (B. D. Nageswara Rao and M. D. Kemple, Eds.), Plenum Press, pp. 251-274 (1996).

235. J. L. Markley, B. Xia, Y. K. Chae, H. Cheng, W. M. Westler, J. D. Pikus, and B. G. Fox, NMR Approaches to the Study of Structure­Function Relationships in Iron­Sulfur Proteins: Rubredoxin, [2Fe­2S] Ferredoxins, and a Rieske Protein, In Protein Structure Function Relationship (Z. Zaidi and D. Smith, Eds.), Plenum Press, London, pp. 135-146 (1996).

236. Z. Dzakula, W. M. Westler, and J. L. Markley, "Continuous Probability Distribution (CUPID) Analysis of Potentials for Internal Rotations," J. Magn. Reson. Ser B 111, 109-126 (1996).

237. Y. K. Chae and J. L. Markley, "Mutagenesis Studies of Anabaena 7120 [2Fe­2S] Ferredoxins: Physical and Chemical Characteristics of Hybrid Vegetative/Heterocyst Sequences," In Photosynthesis: from Light to Biosphere (P. Mathis, Ed.), Kluwer Academic Publishers, Vol. II, 633-638 (1995).

238. A. P. Hinck, W. F. Walkenhorst, D. Truckses, and J. L. Markley, "NMR and Mutagenesis Investigations of a Model Cis:Trans Peptide Isomerization Reaction: Xaa116­Pro117 of Staphylococcal Nuclease and its Role in Protein Folding and Stability," In Biological NMR Spectroscopy (J. L. Markley and S. J. Opella, Eds.), Oxford University Press, 133-160 (1996), in press.

239. Zs. Zolnai, N. Juranic, J. L. Markley, and S. Macura, "Zooming: a Practical Strategy for Improving the Quality of Multidimensional NMR Spectra," J. Magn. Reson. Ser. A. 119, 53-64 (1996).

240. K. E. Prehoda, S. N. Loh, and J. L. Markley, "Modeling Volume Changes in Proteins using Partial Molar Volumes of Model Compounds," In Techniques in Protein Chemistry VII (D. R. Marshak, Ed.), Academic Press, New York, pp 433­438 (1996).

241. Vidugiris, G. A. J., Truckses, D. M., Markley, J. L., and Royer, C. A., "High-Pressure Denaturation of Staphylococcal Nuclease Proline to Glycine Substitution Mutants," Biochemistry 35, 3857-3864 (1996).

242. J. L. Markley and W. M. Westler, "Protonation-State Dependence of Hydrogen Bond Strengths in a Serine Protease Catalytic Triad: Bovine Chymotrypsinogen A," Biochemistry 35, 11092-11097 (1996).

243. C. B. Hoogstraten and J. L. Markley, "Approaches to the Determination of More Accurate Cross-Relaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures," In Dynamics and the Problem of Recognition in Biological Macromolecules (O. Jardetzky and J.-F. Lfevre, Eds.), Plenum Press, pp. 73-111 (1996).

244. C. G. Hoogstraten and J. L. Markley, "Effects of Experimentally Achievable Improvements in the Quality of NMR Distance Constraints on the Accuracy of Calculated Protein Structures, J. Mol. Biol. 258, 334-348 (1996).

245. Truckses, D. M., Somoza, R. R., Prehoda, K. E., and Markley, J. L., "Coupling between cis/trans Proline Isomerization and Protein Stability in Staphylococcal Nuclease," Protein Sci. 5, 1907-1916 (1996).

246. A. P. Hinck, D. M. Truckses, and J. L. Markley, "Engineered Disulfide Bonds in Staphylococcal Nuclease: Effects on Conformational Forms and Stability of the Folded Protein," Biochemistry 35, 10328-10338 (1996).

247. B. Xia, H. Cheng, V. Bandarian, G. H. Reed, and J. L. Markley, "Human Ferredoxin: Overproduction in Escherichia coli, Reconstitution in vitro, and Spectroscopic Studies of Iron-Sulfur Cluster Ligand Cysteine-to-Serine Mutants, Biochemistry 35, 9488-9495 (1996).

248. Z. Dzakula, M. L. DeRider, and J. L. Markley, "Conformational Analysis of Molecules with Five-Membered Rings through NMR Determination of the Continuous Probability Distribution (CUPID) for Pseudorotation," J. Am. Chem. Soc. 118, 12796-12803 (1996).

250. G. Kaslik, J. Kardos, E. Szabó, L. Szilágyi, P. Závodszky, W. M. Westler, J. L. Markley, and L. Gráf, "Effects of Serpin Binding on the Target Proteinase: Global Stabilization, Localized Increased Structural Flexibility, and Conserved Hydrogen Bonding at the Active Site," Biochemistry 29, 5455-5464 (1997).

251. J. Wang, D. M. Truckses, Z. Dzakula, Z. Zolnai, F. Abildgaard, and J. L. Markley, "Three­Dimensional Structures of Staphylococcal Nuclease in Solution from NMR Data: Nuclease H124L and its Ternary Complex with Calcium Ion and Thymidine­3',5'­Bisphosphate, J. Biomol. NMR, in press.

252. P. T. Chivers, K. E. Prehoda, B. F. Volkman, B.-M. Kim, J. L. Markley, and R. T. Raines, "Microscopic pKa Values of Escherichia coli Thioredoxin," Biochemistry, in press.

253. Z. An, Q, Zhao, J. McEvoy, W. M. Yuan, J. L. Markley, and S. A. Leong, "The Second Finger of Urbs1 is Required for Iron-Mediated Repression of sid1 in Ustilago maydis," Proc. Natl. Acad. Sci. USA 94, 5882-5887 (1997).

254. J. L. Markley, Y. Arata, C. W. Hilbers, R. Kaptein, B. D. Sykes, P. E. Wright, and K. Wthrich, "Recommendations for the Presentation of NMR Structures of Proteins and Nucleic Acids," Pure Appl. Chem., in press.

255. J. K. Hurley, A. M. Weber-Main, M. T. Stankovich, M. M. Benning, J. B. Thoden, J. L. Vanhooke, H. M. Holden, Y. K. Chae, B. Xia, H. Cheng, J. L. Markley, C. Genzor, C. Gómez-Moreno, J. L. Schmeits, and G. Tollin, "Structure-Function Relationships in Anabaena Ferredoxin: Correlations Between X-ray Crystal Structures, Redox Potentials, and Rates of Electron Transfer to Ferredoxin:NADP+ Reductase for Site-Specific Ferredoxin Mutants," Biochemistry, in press.

256. S. L. Alam, B. F. Volkman, J. L. Markley, and J. D. Satterlee, "Detailed NMR Analysis of the Heme-Protein Interactions in Component IV-Glycera dibranchiata Monomeric Hemoglobin-CO," J. Biomol. NMR, in press.

257. A. M. Prantner, B. F. Volkman, S. J. Wilkens, B. Xia, and J. L. Markley, "Assignment of 1H, 13C, and 15N Signals of Clostridium pasteurianum Rubredoxin: Oxidation State-Dependent Changes in Chemical Shifts and Relaxation Rates," J. Biomol. NMR., in press.

258. J. Caldwell and J. L. Markley, "Structure-Function Relationships in Sweet-Tasting Proteins," In Protein Structure Function Relationship 97 (Z. Zaidi, Ed.), Plenum Press, in press.