/arsi/volkman/dmx600/README

Instructions on using the setup600.tar file to 
create experiment directories for new users on
the Bruker DMX-600 spectrometer at NMRFAM

Created ????97 BFV
Updated 081298 BFV

Please address questions, problems and comments to
Brian Volkman, volkman@nmrfam.wisc.edu (608)-262-0459
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1.  If you have not used the 600 at all before,
login to dexter, either at the console or from
any NMRFAM workstation.

2. type 'cd /u/data', and then 'ls'

3. if a directory with your username exists, 
type 'cddata'.  'pwd' should show that you are in
the directory /u/data/[username]/nmr. go to #4.

3a. if no such directory exists, create one:
type 'mkdir [username]', and then 
'mkdir [username]/nmr'.  then type 'cddata'.

4.  Make sure you are in the directory
'/u/data/[username]/nmr' (type 'pwd').
Copy the setup600.tar archive to this 
directory (this is your working directory on 
the DMX-600) with the command:

'cp ~volkman/dmx600/setup600.tar .'

5.  unpack the archive: 'tar -xvf setup600.tar'

6.  If you are on the console of dexter (the DMX-600
workstation), type 'xwinnmr' to start the Bruker 
software, if it is not already running.  In the
xwinnmr window, type 'dir' to see the list of basic
experiments which have been installed in your directory.
_________________________________________________

Experiments included in the setup600.tar archive:

-- 1D calibration and tuning

1puls - simple 1 pulse expt; use for tuning 1H channel
	and calibrating 1H pulses.

tune15n - tune 15N channel with wobb

tune13c - tune 13C channel with wobb

tune2h - tune 2H channel with wobb

tune31p - tune 31P channel with wobb

calib15n - calibrate 15N pulse widths on standard (NAG)
	sample (peak at ~ 8 ppm) with decp90-type sequence.  
	water suppression via off-resonance 1-1 sequence.

calib13cf3 - calibrate 15N pulse widths on standard (NAG)
	sample (peak at ~ 2 ppm) with decp90-type sequence.  
	water suppression via off-resonance 1-1 sequence.

-- Basic 1D and 2D 1H, 15N and 13C spectra

1d_watergate - 1D 1H spectrum with 3-9-19 selective inversion/
	watergate-type solvent suppression.  Very robust, should
	require input of only the 1H 90 pulse (p1).

hsgp19_n15 - 2D 15N-1H HSQC experiment with 3-9-19 water suppression
	and water flip-back.  Very robust, should require input 
	of only the 1H 90 pulse (p1).

hsqcse_c13 - 2D 13C-1H HSQC experiment with gradient sensitivity-
	enhancement (Rance/Kay) and magic-angle gradients for 
	improved water suppression.

-- Protein Triple Resonance Experiments

hnco - 3D 1HN-15N-13C' backbone correlation experiment with gradient
	sensitivity enhancement for 15N echo/antiecho quad detection
	and efficient solvent suppression.

hnca - 3D 1HN-15N-13Ca backbone correlation experiment with gradient
	sensitivity enhancement for 15N echo/antiecho quad detection
	and efficient solvent suppression.

hncoca - 3D 1HN-15N-13Ca backbone correlation experiment with gradient
	sensitivity enhancement for 15N echo/antiecho quad detection
	and efficient solvent suppression.

cbcaconh - 3D 1HN-15N-13Ca/b backbone correlation experiment with gradient
	sensitivity enhancement for 15N echo/antiecho quad detection
	and efficient solvent suppression.
______________________________________________________
Please address questions, problems and comments to
Brian Volkman, volkman@nmrfam.wisc.edu (608)-262-0459