NMRFAM STAFF VOLKMAN RESEARCH
NMRFAM

Brian F. Volkman, Ph.D.

DMX Pulse Programs and Parameters

All of these pulse programs were tested on our DMX600, some have been tested on the DMX400, 500 and 750 as well. Original references are included in the text of the pulse program file. For some of the experiments listed, a complete experiment directory from one of the NMRFAM DMX spectrometers is provided as a tar file - look at the README files for help with installation on your spectrometer. I can't promise to provide full support for users outside of NMRFAM, but I will do my best to provide answers for concise, specific questions. Please consult the references given in the pulse program, as in most cases I am simply implementing an experiment which has been fully described in the literature. New users of the NMR facility should consult the NMRFAM Extranet or this brief USER GUIDE (pdf file - open and print from Adobe Acrobat Reader) for answers to general questions on the spectrometers and workstations. If you find errors or have exhausted your local sources of help, email me.

Backbone Triple Resonance

3D/2D HCACO mod. for sidechain carboxyl w/ PFG SE

3D HBHA(CBCACO)NH w/ PFG SE

3D HNCO w/ H2O FB and PFG SE

3D HNCA w/ H2O FB and PFG SE

3D HNCOCA w/ H2O FB and PFG SE

3D HNCACB w/ H2O FB and PFG SE

Quantitative J Correlation*updated!*

3D HNHA for measurement of 3JHN-Ha
Pulse program - hnha.bv
Expt from DMX600 - hnha.tar
Install notes - README

JNC - 2D spin-echo diff CT-HSQCSE for measurement of 3JN-Cg
Pulse program - jnc.bv
Expt from DMX600 - jnc.tar
Install notes - README

JCC - 2D spin-echo diff CT-HSQCSE for measurement of 3JC'-Cg
Pulse program - jcc.bv
Expt from DMX600 - jcc.tar
Install notes - README

15N Relaxation - Backbone Dynamics

15N T2 measurement w/ H2O FB and PFG SE

15N T1 measurement w/ H2O FB and PFG SE

15N-1H heteronuclear NOE w/ H2O FB and PFG SE

Setup Archives: DMX Parameter Sets for Common Experiments

Sets of tested parameter sets for the DMX spectrometers to jump-start NMRFAM users into pulse width calibration and acquisition of 1D, 2D and 3D spectra

setup600.tar - basic experiments for the DMX-600

README-600 - instructions for installing DMX-600 basic experiment archive and list of experiments included

NMRFAM STAFF VOLKMAN RESEARCH